| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RBP10; RPB10; RPABC5; RPB7.6; hRPB7.6; RPB10beta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human POLR2L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于POLR2L抗体的3篇模拟参考文献示例(请注意，以下内容为虚构，建议通过PubMed或SciFinder查询真实文献)：
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1. **文献名称**: "POLR2L as a Novel Biomarker in Breast Cancer: Expression and Clinical Significance"
**作者**: Zhang, Y. et al.
**摘要**: 本研究通过免疫组化分析了POLR2L在乳腺癌组织中的表达水平，发现其高表达与患者预后不良相关。研究利用特异性POLR2L抗体证实了其在肿瘤细胞核内的定位，并揭示了其通过调控RNA聚合酶II活性促进肿瘤进展的机制。
2. **文献名称**: "Structural Insights into the RNA Polymerase II Subunit POLR2L via Cryo-EM"
**作者**: Smith, J.R. et al.
**摘要**: 该研究通过冷冻电镜技术解析了POLR2L在RNA聚合酶II复合体中的三维结构，并开发了高亲和力单克隆抗体用于体外结合实验。结果表明POLR2L对维持聚合酶稳定性至关重要，其抗体可有效阻断体外转录活性。
3. **文献名称**: "Development of a High-Specificity POLR2L Antibody for Neurodegenerative Disease Research"
**作者**: Chen, L. et al.
**摘要**: 本文报道了一种新型兔源多克隆POLR2L抗体的制备与验证。该抗体在Western blot和免疫荧光中表现出高特异性，成功应用于阿尔茨海默病模型小鼠脑组织中RNA聚合酶II亚基的动态变化研究。
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如需真实文献，建议使用关键词“POLR2L antibody”在以下平台检索：
- PubMed (https://pubmed.ncbi.nlm.nih.gov/)
- Google Scholar (https://scholar.google.com)
The POLR2L antibody targets a subunit of RNA polymerase II (RNAP II), a critical enzyme responsible for synthesizing messenger RNA in eukaryotic cells. POLR2L, also known as RPB7. is a conserved core component of RNAP II and plays a role in transcription initiation and elongation. It interacts with other polymerase subunits to stabilize the enzyme complex and may participate in regulating transcription-coupled processes, such as DNA repair or mRNA capping.
Antibodies against POLR2L are widely used in research to study RNAP II assembly, transcriptional regulation, and cellular responses to stress or DNA damage. They are employed in techniques like Western blotting, immunofluorescence (IF), and immunoprecipitation (IP) to assess POLR2L expression, localization, and protein-protein interactions. These antibodies are often produced in rabbits or mice using recombinant POLR2L protein fragments as immunogens.
POLR2L's involvement in transcription makes it a focus in cancer, neurodegenerative diseases, and viral infection studies, where RNAP II dysfunction is implicated. Researchers also use POLR2L antibodies as loading controls in experiments due to its stable expression across cell types. Validation typically includes knockout cell lines or siRNA knockdown to confirm specificity. Commercial antibodies are usually tested for cross-reactivity with related polymerase subunits to ensure accuracy.
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