| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SNAP19 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SNAPC5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **SNAPC5抗体** 的3篇参考文献示例(注：文献为模拟概括，具体引用需核实)：
1. **文献名称**：*The role of SNAPC5 in RNA polymerase III transcription and snRNA biogenesis*
**作者**：Henry, R.W., et al.
**摘要**：研究通过免疫沉淀和Western blot分析SNAPC5在RNA聚合酶III转录复合体中的功能，发现其作为SNAPc亚基对U6 snRNA的启动子识别至关重要，并利用特异性抗体验证其与RNA Pol III的相互作用。
2. **文献名称**：*SNAPC5 deficiency disrupts cellular small nuclear RNA expression and induces apoptosis in human cells*
**作者**：Mittal, V., Hernandez, N.
**摘要**：利用SNAPC5抗体敲低实验，证明SNAPC5缺失导致U1/U2 snRNA表达下降，破坏剪接体功能，进而引发细胞周期阻滞和凋亡，提示其在维持细胞稳态中的作用。
3. **文献名称**：*Structural and functional analysis of the SNAP complex in transcription initiation*
**作者**：Jaworski, E., et al.
**摘要**：通过冷冻电镜和抗体阻断实验，解析SNAPC5在SNAP复合体中的结构定位，揭示其通过与RNA Pol III的特定亚基结合，调控转录起始的分子机制。
**建议**：实际引用时可通过PubMed或Google Scholar搜索关键词“SNAPC5 antibody”、“SNAPC5 function”获取最新文献，并优先选择方法学中明确使用该抗体的研究。
The SNAPC5 antibody targets the SNAPC5 (Small Nuclear RNA Activating Complex Polypeptide 5) protein, a key component of the SNAP complex (also known as the PTF complex). This evolutionarily conserved multiprotein complex is essential for the transcription initiation of small nuclear RNA (snRNA) genes by RNA polymerase III (Pol III). SNAPC5. along with other subunits (SNAPC1-4), binds to the proximal sequence element (PSE) in snRNA gene promoters, facilitating the recruitment of Pol III and coactivators. Structurally, SNAPC5 contains a conserved Myb DNA-binding domain critical for promoter recognition and complex stability.
Antibodies against SNAPC5 are widely used to study snRNA transcription regulation, investigate SNAP complex assembly, and explore its interactions with other transcriptional machinery. They are applied in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map SNAPC5 localization, expression levels, and its role in cellular processes such as splicing and ribosome biogenesis. Dysregulation of SNAPC5 has been implicated in developmental disorders and cancers, making these antibodies valuable tools for mechanistic and biomedical research. Validated SNAPC5 antibodies typically show specificity for human, mouse, or rat homologs, enabling cross-species studies.
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