首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX1含量。GPX1是重要靶点。它是谷胱甘肽过氧化物酶家族成员，能催化还原型谷胱甘肽变成氧化型谷胱甘肽，清除体内过氧化物和自由基，保护细胞免受氧化损伤。在多种疾病尤其癌症、神经退行性疾病研究中，其作用机制是研究热点。试剂盒检测范围为12.5 mIU/mL-800 mIU/mL，适用于多种科研场景，包括但不限于评估氧化应激模型（如肝脏疾病、神经退行性病变等）中酶活性变化、药物或环境因素对抗氧化系统的影响研究，以及代谢性疾病相关机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
缩写：GPX1
Uniprot No.：P04041
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 mIU/mL-800 mIU/mL
灵敏度：3.12 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 86-100 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 8002.2512.2992.2752.119 4001.8451.8341.8401.684 2001.3011.3241.3131.157 1000.8920.8760.8840.728 500.5530.5900.5720.416 250.3950.3810.3880.232 12.50.2510.2340.2430.087 00.1550.1570.156 | ||||||
货期：3-5 working days
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