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产品描述：人丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPT含量。试剂盒检测范围为15.6 uIU/mL-1000 uIU/mL，ALT作为肝脏功能的关键指标，其活性升高常与肝细胞损伤相关，如病毒性肝炎、药物性肝损伤或脂肪肝等病理过程的研究。可为肝脏疾病机制研究、药物肝毒性评估、代谢调控实验等提供可靠数据支持，特别适用于体外研究模型中ALT动态变化的监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AAT1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; Alanine aminotransferase ELISA Kit; ALAT1_HUMAN ELISA Kit; ALT1 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; Glutamic alanine transaminase 1 ELISA Kit; Glutamic pyruvate transaminase (alanine aminotransferase) ELISA Kit; Glutamic pyruvic transaminase 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit; GPT 1 ELISA Kit; gpt ELISA Kit; GPT1 ELISA Kit
缩写：GPT
Uniprot No.：P24298
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 uIU/mL-1000 uIU/mL
灵敏度：3.9 uIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| uIU/mlOD1OD2AverageCorrected 10002.5422.6872.6152.516 5001.7061.7171.7121.613 2501.0031.0891.0460.947 1250.6260.5970.6120.513 62.50.3170.3040.3110.212 31.20.2550.2390.2470.148 15.60.1850.1710.1780.079 00.1030.0950.099 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human ALT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled ALT antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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