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Rabbit Polyclonal ADGRF5 Antibody

  • 中文名: ADGRF5抗体
  • 别    名: GPR116; KPG_001
货号: IPDX13484
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

参考文献

以下是3篇与ADGRF5抗体相关的虚构参考文献示例(内容基于典型研究方向,非真实文献):

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1. **标题**: *ADGRF5 promotes tumor angiogenesis through VEGF signaling in glioblastoma*

**作者**: Li X, et al.

**摘要**: 本研究利用ADGRF5特异性抗体(克隆号:AF5-1C3)发现ADGRF5在胶质母细胞瘤血管内皮细胞中高表达,并通过激活VEGF通路促进肿瘤血管生成,抗体阻断实验显著抑制小鼠模型中肿瘤生长。

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2. **标题**: *Development of a monoclonal antibody targeting the extracellular domain of ADGRF5 for flow cytometry applications*

**作者**: Tanaka K, et al.

**摘要**: 报道了一种针对ADGRF5胞外结构域的新型单克隆抗体(mAb 3E8),经ELISA和流式细胞术验证具有高亲和力,成功应用于检测免疫细胞中ADGRF5的表面表达,揭示其在慢性炎症中的潜在调控作用。

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3. **标题**: *ADGRF5 regulates synaptic plasticity via Gαq-dependent signaling in hippocampal neurons*

**作者**: Müller S, et al.

**摘要**: 通过免疫组织化学(使用兔多抗ADGRF5-pAb#207)和基因敲除技术,发现ADGRF5在海马神经元突触中富集,调控Gαq信号通路影响突触可塑性,为ADGRF5在神经退行性疾病中的作用提供证据。

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注:以上文献为示例性质,实际研究中请通过PubMed或Google Scholar检索真实文献。

背景信息

ADGRF5. also known as GPR116. is a member of the adhesion G protein-coupled receptor (GPCR) family, characterized by its large extracellular N-terminal domains involved in cell-cell and cell-matrix interactions. It plays a role in diverse physiological processes, including lung surfactant homeostasis, metabolic regulation, and cancer progression. ADGRF5 antibodies are essential tools for studying the receptor's expression, localization, and functional mechanisms in tissues and cellular models. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to detect ADGRF5 protein levels or monitor its activation states. Research has linked ADGRF5 dysregulation to diseases like pulmonary alveolar proteinosis, obesity, and breast cancer, underscoring its potential as a therapeutic or diagnostic target. Validated ADGRF5 antibodies are critical for ensuring specificity, particularly given the structural complexity of adhesion GPCRs and their splice variants. Many commercial antibodies target epitopes within the extracellular or intracellular domains, enabling researchers to investigate receptor shedding, signaling pathways, or interactions with ligands like surfactant protein D. Recent studies also explore ADGRF5's role in immune modulation and tissue remodeling, highlighting its broader relevance in pathophysiology. Proper validation using knockout controls or functional assays remains crucial to avoid off-target artifacts in experimental data.

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