首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人磷脂酰肌醇聚糖特异性磷脂酶D(GPLD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPLD1含量。GPLD1（Glycosylphosphatidylinositol-specific phospholipase D1）是一种参与多种生物学过程的酶，包括脂质运输、病原体控制和糖尿病等。其基因敲除小鼠模型已被构建，用于研究其在小鼠体内的功能。研究显示，GPLD1的表达与阿尔茨海默病（AD）模型中β-淀粉样蛋白的产生相关，可能通过调节GAL3BP的表达来影响AD的发生。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研领域中对GPLD1表达动态的定量分析，例如探究其在代谢性疾病、神经系统疾病或肿瘤微环境中的分子机制。提供预包被板条和即用型试剂，操作流程标准化，支持批量样本检测，广泛用于细胞实验、动物模型研究以及生物标志物筛选等场景，为研究GPLD1相关生理病理功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glycoprotein phospholipase D ELISA Kit; Glycosyl-phosphatidylinositol-specific phospholipase D ELISA Kit; GPI-PLD ELISA Kit; GPI-specific phospholipase D ELISA Kit; GPIPLD ELISA Kit; GPIPLDM ELISA Kit; GPLD1 ELISA Kit; MGC22590 ELISA Kit; PHLD_HUMAN ELISA Kit; Phosphatidylinositol glycan specific phospholipase D ELISA Kit; Phosphatidylinositol-glycan-specific phospholipase D ELISA Kit; PI-G PLD ELISA Kit; PIGPLD ELISA Kit; PIGPLD1 ELISA Kit
缩写：GPLD1
Uniprot No.：P80108
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPLD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:1600 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human GPLD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 94-106 | ||||
| EDTA plasma (n=4) | 95 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.8412.7522.7972.6731002.5642.4022.4832.359502.1351.9652.0501.926251.3581.3551.3571.23312.50.6910.7440.7180.5946.250.3710.3950.3830.2593.120.2250.2260.2260.10200.1250.1230.124 | ||||||
货期：3-5 working days
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