| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BDPLT17; ZNF163B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GFI1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GFI1B抗体的3篇参考文献概览：
1. **"Germline GFI1B mutations cause dominant thrombocytopenia and disrupt megakaryocyte development"**
- 作者：Monteferrario D, et al.
- 摘要：该研究首次发现GFI1B基因的种系突变导致常染色体显性血小板减少症，通过GFI1B抗体检测发现突变体影响巨核细胞分化，并破坏靶基因调控网络。
2. **"GFI1B regulates hematopoietic lineage differentiation during embryogenesis"**
- 作者：Saleque S, et al.
- 摘要：利用GFI1B抗体进行染色和功能实验，揭示GFI1B在胚胎造血过程中的关键作用，尤其在红系和巨核系细胞命运决定中的转录调控机制。
3. **"Altered translation of GFI1B in erythroid cells contributes to impaired erythropoiesis"**
- 作者：Sankaran VG, et al.
- 摘要：通过Western blot和免疫荧光(使用GFI1B抗体)发现，红细胞发育异常与GFI1B蛋白翻译异常相关，为贫血疾病提供了分子机制解释。
(注：以上文献为领域内典型研究方向示例，实际文献需通过PubMed等数据库验证具体信息。)
The GFI1B antibody is a critical tool in studying the Growth Factor Independent 1B (GFI1B) protein, a zinc finger transcriptional repressor pivotal in hematopoiesis. GFI1B regulates the development and differentiation of erythroid and megakaryocytic lineages by modulating gene expression through chromatin remodeling. It is essential for maintaining hematopoietic stem cell quiescence and preventing premature differentiation. Mutations in *GFI1B* are linked to disorders like thrombocytopenia, bleeding diatheses, and myelodysplasia, highlighting its role in blood cell homeostasis.
The antibody enables detection of GFI1B in various experimental settings, including Western blotting, immunofluorescence, and flow cytometry, aiding in research on its expression patterns and interactions. Studies using GFI1B antibodies have elucidated its involvement in repressing target genes (e.g., *TAL1*, *GATA1*) and its partnership with co-repressors like LSD1. Dysregulation of GFI1B is also implicated in leukemia, making its antibody valuable in oncology research for probing disease mechanisms or therapeutic targets.
Validating GFI1B antibodies requires specificity checks via knockout controls, as cross-reactivity with homologous proteins (e.g., GFI1) can occur. Its applications span basic research, diagnostic assays for genetic blood disorders, and drug development, underscoring its versatility in both clinical and laboratory contexts.
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