首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人生长分化因子5(GDF5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF5含量。GDF5即生长分化因子5，是TGF -β超家族成员。其在骨骼发育、关节稳态维持等方面有重要作用。研究机制上，它能通过与特定受体结合，激活下游信号通路，影响细胞增殖、分化，在骨关节炎、骨折修复等研究领域受关注。试剂盒检测范围为28 pg/mL-1800 pg/mL，该产品可为骨骼发育机制研究、关节退行性疾病模型构建、软骨再生相关药物筛选等基础研究提供可靠的数据支持，特别适用于需要同时检测多种生物样本类型的实验设计，帮助科研人员系统性解析GDF5在不同生理病理过程中的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP14 ELISA Kit; Cartilage derived morphogenetic protein 1 ELISA Kit; Cartilage-derived morphogenetic protein 1 ELISA Kit; CDMP-1 ELISA Kit; CDMP1 ELISA Kit; GDF-5 ELISA Kit; Gdf5 ELISA Kit; GDF5_HUMAN ELISA Kit; Growth differentiation factor 5 ELISA Kit; Growth/differentiation factor 5 ELISA Kit; LAP4 ELISA Kit; OS5 ELISA Kit; Radotermin ELISA Kit; SYNS2 ELISA Kit
缩写：GDF5
Uniprot No.：P43026
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 103-108 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 90-97 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 91-99 | ||||||
回收率：
| The recovery of human GDF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 97-105 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected18002.6882.7992.7442.6619002.1622.2872.2252.1424501.3741.3991.3871.3042250.7260.7880.7570.674112.50.3520.3630.3580.275560.2370.2440.2410.158280.1440.1500.1470.06400.0800.0850.083 . | |||||||
货期：3-5 working days
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