首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨成型蛋白9(BMP-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GDF2含量。GDF2（生长分化因子2）是一种分泌性的TGF-β超家族蛋白质的配体，其结合TGF-β受体，激活SMAD家族转录因子，调控基因表达。GDF2在软骨和骨发育、血管生成及胆碱能中枢神经系统神经元分化中起重要作用。研究显示，GDF2变异与肺动脉高压（PAH）和遗传性出血性毛细血管扩张症（HHT）相关。试剂盒检测范围为0.312 pg/mL-20 pg/mL，本试剂盒通过高特异性抗体对实现目标蛋白的捕获与检测，操作流程标准化，可在4.5小时内完成检测，为研究BMP - 9在神经生物学、骨骼疾病模型或代谢调控机制中的功能提供可靠工具，广泛适用于细胞实验、动物模型或相关分子通路的基础科研。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gdf2 ELISA Kit; Bmp9Growth/differentiation factor 2 ELISA Kit; GDF-2 ELISA Kit; Bone morphogenetic protein 9 ELISA Kit; BMP-9 ELISA Kit
缩写：GDF2
Uniprot No.：Q9WV56
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 pg/mL-20 pg/mL
灵敏度：0.078 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 91-104 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 97-108 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse BMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 89-103 | ||||
| EDTA plasma (n=4) | 94 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected202.2662.2312.2492.081101.3891.2961.3431.17550.8500.8340.8420.6742.50.5820.5310.5570.3891.250.4220.4410.4320.2640.6250.3160.3030.3100.1420.3120.2490.2330.2410.07300.1700.1660.168 | ||||||
货期：3-5 working days
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