首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠生长分化因子15(GDF15)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF15含量。GDF15是一种生长分化因子。其它在多种组织中表达，与细胞生长、分化等密切相关。研究机制上，它能通过与特定受体结合参与机体能量代谢、炎症反应等调节，在肿瘤、代谢疾病等研究中受关注。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于能量代谢调控机制研究、心血管疾病动物模型评估、肿瘤相关病理分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gdf15 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit
缩写：GDF15
Uniprot No.：Q9Z0J6
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:20 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:80 | Average % | 102 | ||||
| Range % | 99-105 | |||||
回收率：
| The recovery of rat GDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-90 | ||||
| EDTA plasma (n=4) | 93 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average1000.1270.1290.128500.2080.2200.214250.3340.3240.32912.50.4720.4980.4856.250.7130.7350.7243.121.1621.1391.1511.561.5421.5671.55502.8142.8642.839 | ||||||
货期：3-5 working days
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