首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠生长分化因子11(GDF11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF11含量。GDF11是转化生长因子-β超家族成员。其在胚胎发育等过程发挥重要作用。研究机制上，参与调节组织器官的生长、发育与修复，在神经、肌肉等系统有相关作用，近年在衰老研究中受关注，研究其对延缓衰老进程的潜在作用。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研场景中探究GDF11在发育生物学、再生医学及衰老相关疾病模型中的表达动态，例如通过检测不同发育阶段或病理状态下血清、脑组织、肌肉等样本的蛋白水平变化，揭示其在组织稳态维持或再生修复中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gdf11 ELISA Kit; Bmp11Growth/differentiation factor 11 ELISA Kit; GDF-11 ELISA Kit; Bone morphogenetic protein 11 ELISA Kit; BMP-11 ELISA Kit
缩写：GDF11
Uniprot No.：Q9Z1W4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-107 | |||||
回收率：
| The recovery of mouse GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.5312.5982.5652.4186001.9371.9671.9521.8053001.2801.2911.2861.1391500.7100.7630.7370.590750.4300.4650.4480.30137.50.2940.2840.2890.14218.750.2250.2310.2280.08100.1460.1480.147 | ||||||
货期：3-5 working days
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