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产品描述：人生长分化因子11(GDF11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF11含量。GDF11是一种蛋白质，属于转化生长因子-β超家族。在它在胚胎发育等过程中发挥重要作用。研究机制上，其涉及调节细胞生长、分化等，对肌肉、心脏等组织的生长和修复有一定影响，在抗衰老等研究领域受关注。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于探索GDF11在衰老机制、组织再生、代谢调控等基础研究领域的动态表达规律，亦可用于心血管疾病、神经退行性疾病等模型中的蛋白水平分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP 11 ELISA Kit; BMP-11 ELISA Kit; BMP11 ELISA Kit; Bone morphogenetic protein 11 ELISA Kit; GDF 11 ELISA Kit; GDF-11 ELISA Kit; Gdf11 ELISA Kit; GDF11_HUMAN ELISA Kit; Growth differentiation factor 11 ELISA Kit; Growth/differentiation factor 11 ELISA Kit
缩写：GDF11
Uniprot No.：O95390
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-93 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 83-91 | |||||
回收率：
| The recovery of human GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-98 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.6992.7522.7262.5799001.8791.9061.8931.7464501.1211.2001.1611.0142250.5480.5450.5470.400112.50.3060.3320.3190.172560.2250.2380.2320.085280.2130.2040.2090.06200.1510.1420.147 | ||||||
本试剂盒所含材料：
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A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GDF11 This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GDF11 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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