| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human EIF2S1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于EIF2S1抗体的参考文献示例(内容基于假设性研究，仅供参考)：
---
1. **文献名称**：*Phosphorylation of eIF2α in Alzheimer's disease brain models*
**作者**：M. Costa-Mattioli et al.
**摘要**：研究通过使用EIF2S1抗体(特异性识别磷酸化形式)发现，阿尔茨海默病小鼠模型的海马组织中eIF2α磷酸化水平显著升高，提示其与神经元内质网应激及tau蛋白异常聚集相关。
---
2. **文献名称**：*Viral infection modulates eIF2α signaling via EIF2S1 antibodies*
**作者**：A. Pizzirusso et al.
**摘要**：利用EIF2S1抗体分析病毒感染后宿主细胞的应激反应，发现甲型流感病毒通过激活PKR激酶诱导eIF2α磷酸化，进而抑制病毒蛋白翻译，揭示宿主抗病毒机制的关键调控节点。
---
3. **文献名称**：*EIF2S1 mutations disrupt translation initiation in cancer*
**作者**：T. Yoshizawa et al.
**摘要**：通过免疫组化(EIF2S1抗体)和基因测序，发现部分肝癌患者存在EIF2S1基因突变，导致eIF2α功能异常，促进肿瘤微环境中的异常蛋白质合成及细胞增殖。
---
4. **文献名称**：*Dynamic regulation of eIF2α phosphorylation during ER stress*
**作者**：S. Walter et al.
**摘要**：研究利用EIF2S1特异性抗体追踪内质网应激过程中eIF2α磷酸化动力学，发现PERK-eIF2α-ATF4通路在维持细胞存活与凋亡平衡中的时间依赖性调控机制。
---
注：以上文献为示例性内容，实际引用时需查询真实数据库(如PubMed)并核实信息准确性。
**Background of EIF2S1 Antibody**
EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1), also known as eIF2α, is a critical component of the eukaryotic translation initiation factor 2 (eIF2) complex, which regulates protein synthesis initiation. The eIF2 complex delivers methionyl-tRNA to ribosomes during the formation of the 43S preinitiation complex, a key step in mRNA translation. EIF2S1. the alpha subunit of eIF2. plays a pivotal role in cellular stress responses. Under stress conditions (e.g., nutrient deprivation, viral infection, or ER stress), kinases such as PERK, GCN2. PKR, or HRI phosphorylate EIF2S1 at Ser51. converting eIF2 from a substrate to an inhibitor of its guanine nucleotide exchange factor, eIF2B. This phosphorylation reduces global protein synthesis while selectively enhancing the translation of stress-responsive mRNAs, such as ATF4. to restore cellular homeostasis.
Antibodies targeting EIF2S1 are widely used to study translational regulation, stress signaling pathways (e.g., the integrated stress response), and diseases linked to dysregulated protein synthesis, including neurodegeneration, cancer, and metabolic disorders. These antibodies detect total EIF2S1 or its phosphorylated form (p-eIF2α), enabling insights into cellular stress status. They are essential tools in Western blotting, immunofluorescence, and immunohistochemistry, helping researchers explore mechanisms of gene expression control, therapeutic interventions, and biomarker discovery.
×
