首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠葡萄糖激酶(GCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GCK含量。GCK即葡萄糖激酶，在糖代谢调节中起关键作用，是糖代谢的感受器。研究机制层面，它能催化葡萄糖磷酸化，影响胰岛素分泌与肝脏糖原合成。鉴于其在血糖调控中的重要地位，GCK成为糖尿病等代谢疾病药物研发的热门靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于糖尿病机制研究、代谢性疾病动物模型评估、体外细胞糖代谢调控实验及药物干预效果分析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gck ELISA Kit; Gk ELISA Kit; Hexokinase-4 ELISA Kit; HK4 ELISA Kit; EC 2.7.1.1 ELISA Kit; Glucokinase ELISA Kit; Hexokinase type IV ELISA Kit; HK IV ELISA Kit; Hexokinase-D ELISA Kit
缩写：GCK
Uniprot No.：P52792
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 87-99 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 86-98 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 82-97 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 90-105 | ||||||
回收率：
| The recovery of mouse GCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 92-105 | |||||
| EDTA plasma (n=4) | 104 | 95-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.2522.1562.2042.026 5001.5881.5291.5591.381 2501.0321.0841.0580.880 1250.6870.6620.6750.497 62.50.5150.5280.5220.344 31.250.4260.4350.4310.253 15.60.3000.3110.3060.128 00.1800.1760.178 | |||||||
货期：3-5 working days
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