首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰高血糖素(GC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GCG含量。GCG是人体中能编码胰高血糖素等激素的基因。其编码产物在维持血糖稳态等方面发挥关键作用，当GCG功能异常会影响血糖调节。研究上主要聚焦其信号通路、与疾病关联机制，期望以此为靶点开发治疗糖尿病、肥胖症等代谢疾病药物。试剂盒检测范围为0.78 pg/mL-50 pg/mL，可广泛用于动物模型研究、代谢调控机制探索、药物干预效果评估等科研领域。样本兼容性覆盖血液（血清/血浆）及组织提取物，为多维度实验设计提供支持，例如探究不同生理状态下胰高血糖素分泌动态或器官特异性表达差异。助力研究人员深入解析GC在能量代谢网络中的功能及病理生理学意义。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GcgGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA kit; GRPP); Oxyntomodulin ELISA kit; OXM ELISA kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA kit; GLP-2)] ELISA kit
缩写：GCG
Uniprot No.：P06883
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 94 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected502.0352.0222.0291.890251.3301.3211.3261.18712.50.9040.8920.8980.7596.250.5540.5620.5580.4193.120.3600.3560.3580.2191.560.2780.2870.2830.1440.780.2390.2290.2340.09500.1410.1370.139 | ||||||
货期：3-5 working days
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