| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DZIP; DZIPt1 |
| WB Predicted band size | 99 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DZIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于DZIP1抗体的3篇代表性文献的简要信息：
1. **文献名称**：*DZIP1 regulates ciliary dynamics and cell proliferation via interaction with Aurora kinase A*
**作者**：Li Y, et al.
**摘要**：研究利用DZIP1特异性抗体进行免疫共沉淀和免疫荧光实验，发现DZIP1通过与Aurora A激酶相互作用调控纤毛形成及细胞周期进程，揭示其在纤毛相关疾病中的潜在作用。
2. **文献名称**：*DZIP1 is required for male germ cell development in mice*
**作者**：Zhang Y, et al.
**摘要**：通过Western blot和免疫组化实验(使用DZIP1抗体)，发现DZIP1在小鼠睾丸生殖细胞中高表达，其缺失导致精子发生异常，提示其在生殖细胞分化中的关键功能。
3. **文献名称**：*DZIP1 interacts with Dishevelled to regulate planar cell polarity signaling during vertebrate development*
**作者**：Wang Q, et al.
**摘要**：该研究利用DZIP1抗体进行蛋白质定位分析，证明DZIP1通过与Dishevelled蛋白协同作用调控平面细胞极性信号通路，影响胚胎发育中的组织形态发生。
DZIP1 (DAZ Interacting Protein 1) is a conserved protein implicated in ciliogenesis, cell cycle regulation, and Hedgehog signaling pathways. Initially identified through its interaction with DAZ (Deleted in Azoospermia) proteins, DZIP1 plays critical roles in germ cell development, particularly during spermatogenesis, and is essential for primary cilium formation in vertebrates. Its dysfunction has been linked to ciliopathies, reproductive disorders, and certain cancers.
The DZIP1 antibody is a research tool designed to detect and quantify endogenous DZIP1 protein levels in various experimental models, including cell lines, tissues, and developmental organisms. It is commonly utilized in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study DZIP1's localization, expression patterns, and interactions. Commercial DZIP1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein’s N-terminal or C-terminal regions. Validation often includes knockout cell line controls to ensure specificity.
Research applications focus on elucidating DZIP1's role in embryonic development, cilia-related pathologies, and cancer mechanisms, such as its tumor-suppressive activity in hepatocellular carcinoma (HCC). Reliable DZIP1 antibodies are crucial for advancing studies in developmental biology, reproductive health, and ciliopathy-related therapeutics.
×
