| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DUSP27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是模拟生成的关于DUSP27抗体的参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**：*Characterization of a Novel Monoclonal Antibody Against DUSP27 and Its Application in Skeletal Muscle Studies*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种针对DUSP27蛋白的单克隆抗体，验证了其在人和小鼠骨骼肌组织中的特异性。通过免疫组化和Western blot分析，发现DUSP27在肌肉再生过程中表达上调，提示其可能参与肌肉分化调控。
2. **文献名称**：*DUSP27 Expression in Testicular Cells: Insights from a Polyclonal Antibody-Based Approach*
**作者**：Lee H, et al.
**摘要**：利用新开发的多克隆抗体，揭示了DUSP27在哺乳动物睾丸组织中的定位，发现其在精子发生早期阶段高表达，暗示其可能通过调控MAPK信号通路影响生殖细胞发育。
3. **文献名称**：*DUSP27 Antibody Validation for Metabolic Disease Models*
**作者**：Garcia R, et al.
**摘要**：通过抗体验证实验证实了DUSP27在肝脏组织中的表达与肥胖模型中的胰岛素抵抗相关，提示该抗体可作为代谢疾病研究的潜在工具。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。如需进一步协助文献检索，请提供更多具体信息。
The DUSP27 antibody is designed to target dual-specificity phosphatase 27 (DUSP27), also known as VHP or DUSP25. a member of the protein tyrosine phosphatase (PTP) family. DUSP27 is classified as an atypical dual-specificity phosphatase due to its unique structure and substrate specificity. Unlike other DUSPs that primarily regulate mitogen-activated protein kinase (MAPK) signaling, DUSP27 lacks the conserved N-terminal MAPK-binding domain, suggesting divergent regulatory roles. It is predominantly expressed in tissues such as the heart, skeletal muscle, and kidney, and has been implicated in cellular processes like oxidative stress response, insulin signaling, and metabolic regulation. Studies indicate its potential involvement in diabetes, cardiovascular diseases, and cancer, though its precise molecular mechanisms remain under investigation.
Antibodies against DUSP27 are essential tools for elucidating its biological functions. They enable the detection and quantification of DUSP27 expression in various experimental models via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Polyclonal or monoclonal DUSP27 antibodies, often raised in rabbits or mice, are validated for specificity to ensure minimal cross-reactivity with other phosphatases. Recent research has utilized these antibodies to explore DUSP27's role in disease contexts, such as its downregulation in diabetic cardiomyopathy or aberrant expression in certain tumors. Ongoing efforts aim to clarify its interactions with substrates and pathways, positioning DUSP27 as a potential therapeutic target. The development of reliable antibodies continues to advance our understanding of its physiological and pathological significance.
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