首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰高血糖素(GC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GCG含量。GCG 即胰高血糖素基因，其编码的胰高血糖素在血糖调节等方面发挥关键作用。研究机制上，主要围绕其与受体结合激活信号通路，调节肝糖输出、脂肪代谢等。对 GCG 的深入研究有助于理解代谢相关疾病发病机理并开发新疗法。试剂盒检测范围为12.9 pg/mL-200 pg/mL，适用于科研领域探究糖代谢调控机制、评估疾病模型动物GC分泌动态、筛选调节GC通路的化合物等研究，为糖尿病病理分析、代谢性疾病基础研究及药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GCG ELISA Kit; Glicentin related polypeptide ELISA Kit; glicentin-related polypeptide ELISA Kit; GLP-1 ELISA Kit; GLP-1(7-36) ELISA Kit; GLP-1(7-37) ELISA Kit; GLP-2 ELISA Kit; GLP1 ELISA Kit; GLP1; included ELISA Kit; GLP2 ELISA Kit; GLP2; included ELISA Kit; GLUC_HUMAN ELISA Kit; Glucagon ELISA Kit; Glucagon like peptide 1 ELISA Kit; glucagon-like peptide 1 ELISA Kit; Glucagon-like peptide 1; included ELISA Kit; Glucagon-like peptide 2 ELISA Kit; Glucagon-like peptide 2; included ELISA Kit; GRPP ELISA Kit; OXM ELISA Kit; OXY ELISA Kit; preproglucagon ELISA Kit
缩写：GCG
Uniprot No.：P01275
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.9 pg/mL-200 pg/mL
灵敏度：4.3 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 99 | 93-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2002.5342.5522.5432.514 1001.3121.2761.2941.265 57.20.6420.6140.6280.599 28.60.2710.2590.2650.236 12.90.1220.1240.1230.094 00.0280.0290.029 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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