首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔纤连蛋白(FN1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FN1含量。FN1即纤连蛋白 1，是细胞外基质的重要成分。它参与细胞黏附、迁移、增殖等多种生理过程。在研究机制方面，FN1 可与多种受体相互作用，调节细胞信号通路。其异常表达与肿瘤、纤维化等疾病密切相关，是潜在治疗靶点。试剂盒检测范围为1.25 ng/mL-20 ng/mL，适用于实验室对FN1蛋白表达水平的动态监测，可广泛用于心血管疾病模型、纤维化机制研究、肿瘤微环境分析等基础科研领域，为探索细胞外基质调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FN1Fibronectin ELISA Kit; FN ELISA Kit; Fragment ELISA Kit
缩写：FN1
Uniprot No.：Q28749
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 98-108 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of rabbit FN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 96-101 | ||||
| EDTA plasma (n=4) | 89 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average200.1500.1600.155100.2680.2870.27850.5520.5660.5592.50.9210.9220.9221.251.4771.4241.45102.6622.5812.622 | ||||||
货期：3-5 working days
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