| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBOX6; WDSAM1 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WDSUB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WDSUB1抗体的模拟参考文献示例(注：以下内容为虚构，仅用于格式参考，实际文献需通过学术数据库检索验证)：
1. **文献名称**: "WDSUB1 regulates neural crest migration via ubiquitination signaling"
**作者**: Zhang L, et al.
**摘要**: 本研究利用兔源多克隆WDSUB1抗体，通过免疫沉淀和Western blot技术，揭示了WDSUB1在神经嵴细胞迁移中通过调控泛素化通路的作用机制。
2. **文献名称**: "Characterization of WDSUB1 expression in hepatocellular carcinoma"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化及小鼠单克隆WDSUB1抗体，研究发现WDSUB1在肝癌组织中高表达，并与患者预后不良相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: "WDSUB1 interacts with β-catenin in Wnt signaling pathway"
**作者**: Kim S, et al.
**摘要**: 采用人源WDSUB1单克隆抗体进行共聚焦显微镜分析，证实WDSUB1与β-catenin存在直接相互作用，影响Wnt信号通路的活性及胚胎发育过程。
4. **文献名称**: "Development and validation of a specific WDSUB1 antibody for zebrafish models"
**作者**: Gupta R, et al.
**摘要**: 报道了一种针对斑马鱼WDSUB1蛋白的多克隆抗体的开发与验证，通过免疫印迹和全胚胎染色，证明了其在体内外实验中的特异性应用。
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**注意**：以上文献信息为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词如“WDSUB1 antibody”或“WDSUB1 immunohistochemistry”)。若该蛋白研究较少，可扩展至其功能相关领域或查看抗体供应商(如CST、Abcam)提供的引用文献列表。
WDSUB1 (WD repeat-containing protein SUB1) is a member of the WD40 repeat protein family, characterized by conserved structural motifs involved in protein-protein interactions. It plays roles in transcriptional regulation, chromatin remodeling, and cellular signaling pathways. The WDSUB1 antibody is a laboratory-generated tool designed to detect and study the expression, localization, and functional mechanisms of the WDSUB1 protein in various biological contexts.
This antibody is typically produced by immunizing animals (e.g., rabbits or mice) with a synthetic peptide or recombinant protein corresponding to a specific epitope of WDSUB1. It has been validated for applications such as Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). Researchers use it to investigate WDSUB1's involvement in cellular processes like cell cycle control, DNA repair, and differentiation, as well as its potential dysregulation in diseases such as cancer or neurodegenerative disorders.
The WDSUB1 antibody’s specificity is confirmed through knockout/knockdown controls or competitive assays. Its development supports mechanistic studies exploring how WDSUB1 interacts with partner proteins (e.g., transcription factors or histone modifiers) to regulate gene expression. As WD40 proteins often serve as scaffolds for macromolecular complexes, this antibody aids in mapping WDSUB1’s role in maintaining structural and functional integrity of such complexes.
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