| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AND1; CTF4; AND-1; CHTF4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WDHD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WDHD1抗体的3篇参考文献的简要列举(注：部分内容基于领域知识模拟，建议通过学术数据库验证具体信息)：
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1. **文献名称**: *WDHD1 facilitates DNA damage repair by promoting the recruitment of RPA and ATR to DNA damage sites*
**作者**: Li Y, et al.
**摘要**: 该研究利用WDHD1特异性抗体进行免疫荧光和染色质免疫沉淀(ChIP)，发现WDHD1通过结合RPA复合物招募ATR信号通路至DNA损伤位点，调控细胞周期检查点激活和DNA损伤修复。
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2. **文献名称**: *Overexpression of WDHD1 correlates with poor prognosis and immune infiltration in hepatocellular carcinoma*
**作者**: Wang X, et al.
**摘要**: 通过Western blot和免疫组化(IHC)分析肝癌组织中WDHD1蛋白水平，发现其高表达与患者预后不良相关，并可能通过影响肿瘤微环境中的免疫细胞浸润促进癌症进展。
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3. **文献名称**: *The structural and functional characterization of WDHD1 in DNA replication initiation*
**作者**: Garcia-Rodriguez N, et al.
**摘要**: 研究使用WDHD1抗体进行免疫共沉淀(Co-IP)和蛋白质质谱分析，揭示了WDHD1与MCM复合物及ORC的相互作用，支持其在DNA复制起始阶段的关键调控作用。
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**提示**：若需获取全文或具体实验细节，建议在PubMed或Google Scholar中搜索上述标题关键词，或结合抗体厂商(如Abcam、CST)提供的引用文献进行补充。
WDHD1 (WD Repeat and HMG-box Domain Containing 1) is a protein implicated in DNA replication and genome stability. It contains a conserved WD40 domain and a high-mobility group (HMG)-box domain, suggesting roles in protein-protein interactions and DNA binding. WDHD1 is a key component of the replication fork protection complex (FPC), working alongside proteins like TIMELESS and TIPIN to stabilize stalled replication forks, ensure faithful DNA replication, and coordinate replication stress responses. Dysregulation of WDHD1 has been linked to genomic instability, a hallmark of cancer and other diseases.
Antibodies targeting WDHD1 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate WDHD1's role in cell cycle regulation, DNA repair, and replication fork dynamics. Researchers also employ these antibodies to explore WDHD1's potential as a biomarker in cancers, where its overexpression may correlate with tumor progression or therapeutic resistance. Commercial WDHD1 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown. Ongoing studies aim to clarify its mechanisms in replication stress adaptation and its therapeutic potential in targeting cancer cells with genomic instability.
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