| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | eNOS; ECNOS |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NOS3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NOS3(eNOS)抗体的参考文献及其摘要概括：
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1. **标题**: "Localization of endothelial nitric oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme"
**作者**: Fulton D. et al.
**摘要**: 研究利用特异性NOS3抗体，通过免疫荧光和Western blot分析eNOS在血管内皮细胞中的亚细胞定位，揭示了磷酸化修饰(Ser1179)如何影响酶活性及NO释放。
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2. **标题**: "Regulation of endothelium-derived nitric oxide production by the protein kinase Akt"
**作者**: Dimmeler S. et al.
**摘要**: 通过抗体验证eNOS蛋白在Akt激酶调控下的磷酸化机制，证明Akt介导的eNOS激活可增强内皮细胞中NO生成，为心血管保护机制提供证据。
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3. **标题**: "Subcellular targeting and trafficking of nitric oxide synthases"
**作者**: Michel T. & Feron O.
**摘要**: 综述eNOS抗体在亚细胞定位研究中的应用，探讨eNOS在细胞膜、高尔基体等区域的动态分布及其与疾病(如动脉粥样硬化)的关联。
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4. **标题**: "eNOS deficiency acts through endothelin to aggravate sFlt-1-induced pre-eclampsia-like phenotype"
**作者**: Li F. et al.
**摘要**: 利用eNOS抗体检测基因敲除小鼠模型中的蛋白表达缺失，发现eNOS缺陷通过内皮素途径加剧妊娠高血压症状，提示其病理生理学中的关键作用。
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这些文献涵盖了NOS3抗体的应用场景(如定位、功能研究、疾病模型分析)，可作为相关研究的参考基础。
NOS3 (Nitric Oxide Synthase 3), also known as endothelial nitric oxide synthase (eNOS), is an enzyme encoded by the *NOS3* gene in humans. It plays a critical role in vascular homeostasis by catalyzing the production of nitric oxide (NO), a signaling molecule that regulates vasodilation, blood flow, and endothelial function. Dysregulation of NOS3 activity or expression is implicated in cardiovascular diseases, hypertension, diabetes, and atherosclerosis, making it a key research target in vascular biology and pathophysiology.
NOS3 antibodies are immunological tools designed to detect and quantify the expression, localization, and post-translational modifications of the eNOS protein in experimental models. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and ELISA to study eNOS expression patterns in tissues, particularly endothelial cells. Researchers also employ them to investigate mechanisms underlying endothelial dysfunction, oxidative stress, and NO signaling pathways. Specific NOS3 antibodies may target distinct epitopes or phosphorylation sites (e.g., Ser1177 or Thr495) to assess activation states, aiding in the evaluation of therapeutic interventions targeting vascular health. Validation of NOS3 antibodies is essential, as cross-reactivity with other NOS isoforms (e.g., neuronal nNOS/NOS1 or inducible iNOS/NOS2) can occur. Their applications extend to both basic research and preclinical studies, offering insights into diseases linked to impaired NO bioavailability.
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