首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠凝血酶原片段F1+2(F1+2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F1+2含量。试剂盒检测范围为62.5 pmol/L-4000 pmol/L，适用于研究凝血与纤溶系统失衡相关疾病机制，如血栓形成模型、抗凝药物药效评价或血管内皮损伤实验中的凝血激活状态监测。科研人员可通过本试剂盒精准量化大鼠模型在病理生理条件下凝血酶原活化水平，为心血管疾病、弥散性血管内凝血（DIC）等研究提供可靠数据支持，但不可用于临床诊断或治疗监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F1+2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pmol/L-4000 pmol/L
灵敏度：15.6 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/LOD1OD2AverageCorrected40002.7392.7262.7332.62220002.2432.2012.2222.11110001.4471.4411.4441.3335000.8020.7390.7710.6602500.4270.4140.4210.3101250.2690.2520.2610.15062.50.1780.1660.1720.06100.1130.1090.111 | ||||||
货期：3-5 working days
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