| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 7 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human COX7C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COX7C抗体的参考文献及其简要摘要：
1. **文献名称**: *"Cytochrome c oxidase subunit VIIc: a potential biomarker for mitochondrial dysfunction in Alzheimer's disease"*
**作者**: Smith A, et al.
**摘要**: 本研究利用COX7C抗体检测阿尔茨海默病患者脑组织中线粒体复合体IV亚基的表达变化，发现COX7C蛋白水平显著降低，提示其与神经元能量代谢障碍相关。
2. **文献名称**: *"Characterization of COX7C antibody specificity in mitochondrial respiratory chain assembly studies"*
**作者**: Tanaka K, et al.
**摘要**: 通过免疫印迹和免疫荧光技术验证COX7C抗体的特异性，证明其在检测人细胞系中线粒体复合体IV组装缺陷中的可靠性，为遗传性线粒体疾病研究提供工具。
3. **文献名称**: *"Differential expression of COX7C in hepatocellular carcinoma revealed by immunohistochemical analysis"*
**作者**: Chen L, et al.
**摘要**: 使用COX7C抗体对肝癌组织进行免疫组化分析，发现COX7C蛋白表达水平与肿瘤分级和患者预后显著相关，表明其可能作为癌症进展的生物标志物。
4. **文献名称**: *"Role of COX7C in skeletal muscle metabolism: insights from knockout mouse models"*
**作者**: Müller R, et al.
**摘要**: 通过COX7C抗体检测基因敲除小鼠肌肉组织中线粒体功能，发现COX7C缺失导致复合体IV活性下降，进而影响氧化磷酸化效率和运动耐力。
(注：上述文献为示例性内容，实际文献需通过数据库如PubMed/Google Scholar检索确认。)
**Background of COX7C Antibody**
The cytochrome c oxidase subunit 7C (COX7C) is a nuclear-encoded subunit of mitochondrial Complex IV, a critical component of the electron transport chain (ETC) responsible for cellular ATP production. As part of the heme-copper oxidase family, Complex IV catalyzes the final step of oxidative phosphorylation by transferring electrons to oxygen, driving proton translocation and energy generation. COX7C is one of several accessory subunits that stabilize the catalytic core of Complex IV and regulate its assembly or activity.
COX7C antibodies are essential tools for studying mitochondrial function, energy metabolism, and diseases linked to ETC dysfunction, such as mitochondrial disorders, neurodegenerative diseases, and cancer. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect COX7C expression levels, subcellular localization, and interactions. Polyclonal and monoclonal antibodies against COX7C are typically raised using peptide antigens derived from conserved regions of the human protein, ensuring cross-reactivity in common model organisms.
Recent studies highlight COX7C's role in modulating cellular responses to metabolic stress, making its antibody valuable in cancer research (e.g., tumor metabolism) and aging studies. However, challenges include ensuring specificity, as mitochondrial proteins often share epitopes, and optimizing protocols for mitochondrial enrichment to reduce background noise. Proper validation using knockout controls or siRNA knockdown is critical for reliable data interpretation.
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