| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FAS; OA-519; SDR27X1 |
| WB Predicted band size | 273 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human FASN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于FASN抗体的3篇示例参考文献(内容为虚构示例，实际引用请查询真实数据库)：
---
1. **文献名称**: *FASN overexpression in breast cancer: a therapeutic target explored via monoclonal antibody inhibition*
**作者**: Smith J, et al.
**摘要**: 研究通过Western blot和免疫组化证实FASN在乳腺癌组织中的高表达，并开发了一种特异性单克隆抗体(mAb-3C2)，体外实验显示其可抑制肿瘤细胞增殖并诱导凋亡。
2. **文献名称**: *Anti-FASN antibody attenuates lipid accumulation in obesity models*
**作者**: Chen L, et al.
**摘要**: 利用FASN多克隆抗体(pAb-F12)在小鼠高脂饮食模型中验证其作用，结果显示抗体处理显著减少肝脏脂质沉积，提示FASN抗体可能改善代谢综合征相关病理。
3. **文献名称**: *Development of a high-affinity FASN antibody for diagnostic applications*
**作者**: Kim S, et al.
**摘要**: 报道一种新型兔源FASN单克隆抗体(Clone R4A)的开发，该抗体在ELISA和免疫荧光中表现出高特异性，可用于癌症患者血清FASN水平的检测，具有潜在临床诊断价值。
---
**提示**：实际文献需通过PubMed、Google Scholar等平台搜索关键词(如“FASN antibody”, “Fatty Acid Synthase inhibitor”)获取，并注意筛选近年高影响力期刊(如*Nature Metabolism*, *Cancer Research*等)。
Fatty acid synthase (FASN) is a multifunctional enzyme complex critical for de novo lipogenesis, catalyzing the synthesis of saturated fatty acids from acetyl-CoA and malonyl-CoA. Overexpression of FASN is observed in various cancers and metabolic disorders, where it supports rapid cell proliferation, membrane synthesis, and energy storage. FASN antibodies are essential tools for studying its expression, localization, and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify FASN levels in tissues or cell lines, aiding research on cancer progression, obesity, and insulin resistance.
Monoclonal FASN antibodies offer high specificity, often targeting distinct epitopes within the enzyme's functional domains (e.g., β-ketoacyl synthase or thioesterase regions), while polyclonal antibodies may detect multiple regions, enhancing sensitivity. Validation via knockout controls or siRNA silencing ensures antibody reliability. Clinically, FASN inhibitors are under investigation as anticancer therapies, making FASN antibodies pivotal for pharmacodynamic studies and biomarker validation. However, cross-reactivity with homologous proteins in certain species requires careful validation. Overall, FASN antibodies remain indispensable for unraveling lipid metabolism dysregulation in disease and exploring targeted therapeutic strategies.
×
