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Human Eosinophil peroxidase(EPX) ELISA kit

  • 中文名: 人嗜酸性粒细胞过氧化酶(EPX)ELISA kit
货号: IPD-EL007756HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:人嗜酸性粒细胞过氧化酶(EPX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液、组织匀浆样本中的EPX含量。人嗜酸性粒细胞过氧化酶(EPX)是一种存在于嗜酸性粒细胞中的酶,参与炎症反应和细胞损伤。其功能包括产生氧化应激和破坏细胞外基质,在嗜酸性粒细胞增多相关疾病(如嗜酸性粒细胞性食管炎)的发病机制中起重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL,适用于体外实验和基础研究中EPX表达水平的动态监测,例如炎症模型构建、药物作用评估或细胞因子网络调控分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Eosinophil peroxidase [Precursor] ELISA Kit; Eosinophil peroxidase ELISA Kit; Eosinophil peroxidase heavy chain ELISA Kit; EPER ELISA Kit; EPO ELISA Kit; EPP ELISA Kit; EPX ELISA Kit; EPX PEN ELISA Kit; PERE ELISA Kit; PERE_HUMAN ELISA Kit

  • 缩写:EPX

  • Uniprot No.:P11678

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, cell culture supernates, tissue homogenates

  • 检测范围:3.12 ng/mL-200 ng/mL

  • 灵敏度:0.78 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Cancer

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human EPX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %87
    Range %80-95
    1:2Average %95
    Range %90-100
    1:4Average %92
    Range %87-99
    1:8Average %97
    Range %92-102
  • 回收率:

    The recovery of human EPX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9086-96
    EDTA plasma (n=4)9590-101














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected 2001.9521.8721.9121.818 1001.4111.3851.3981.304 500.8420.8040.8230.729 250.4440.4190.4320.338 12.50.2720.2540.2630.169 6.250.1810.1690.1750.081 3.120.1310.1210.1260.032 00.0970.0910.094  






  • 货期:3-5 working days

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