| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ANX3 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ANXA3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WWOX抗体的3篇参考文献及其简要摘要：
1. **"WWOX gene expression and protein localization in human breast cancer"**
- **作者**: Del Mare, S., et al. (2011)
- **摘要**: 该研究利用WWOX抗体通过免疫组化分析乳腺癌组织样本，发现WWOX蛋白在癌变组织中显著缺失，且其表达水平与肿瘤恶性程度呈负相关，提示其在乳腺癌抑制中的潜在作用。
2. **"WWOX interacts with p73 to regulate pro-apoptotic function in cancer cells"**
- **作者**: Abdeen, S.K., et al. (2011)
- **摘要**: 研究通过Western blot和免疫共沉淀技术(使用WWOX特异性抗体)，揭示了WWOX与p73蛋白的相互作用，并证明其协同诱导癌细胞凋亡的分子机制。
3. **"Loss of WWOX expression in human cancers: A tumor suppressor role in multiple malignancies"**
- **作者**: Hussain, S., et al. (2015)
- **摘要**: 该文献系统分析了多种癌症(如肺癌、卵巢癌)中WWOX蛋白的表达缺失现象(基于抗体检测)，并探讨了其作为泛癌种肿瘤抑制因子的临床意义。
这些文献均通过WWOX抗体开展实验，聚焦其在肿瘤发生、蛋白互作及临床诊断中的应用。如需扩展，可补充早期克隆鉴定研究(如Bednarek et al., 2000)。
The WWOX (WW Domain-Containing Oxidoreductase) gene, located on chromosome 16q23.1. encodes a tumor suppressor protein involved in regulating cellular processes such as apoptosis, DNA damage response, and metabolic homeostasis. The WWOX protein contains two WW domains that mediate protein-protein interactions and a short-chain dehydrogenase/reductase (SDR) domain, suggesting roles in redox regulation. Dysregulation of WWOX has been linked to cancer progression, neurodegenerative disorders, and metabolic diseases. Its frequent loss of heterozygosity or reduced expression in cancers (e.g., breast, ovarian, lung) underscores its tumor-suppressive function.
WWOX antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Researchers use these antibodies to explore WWOX's role in pathways like Hippo, Wnt/β-catenin, and ERK signaling. Specific antibodies may target distinct epitopes (e.g., N-terminal, C-terminal) or post-translational modifications. Validation using WWOX-knockout cell lines or tissues is critical to ensure specificity, as commercial antibodies vary in performance. Recent studies also highlight WWOX's involvement in neurological conditions (e.g., epilepsy, Alzheimer's) and metabolic syndromes, expanding its research relevance. High-quality WWOX antibodies are pivotal for advancing mechanistic insights and therapeutic targeting in disease models.
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