| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | OODD; SSPS; STHAG4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human WNT10A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WNT10A抗体的3篇参考文献示例(注：以下为示例性内容，实际文献需通过学术数据库核实)：
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1. **文献名称**: *WNT10A mutations cause ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation*
**作者**: Adaimy, L., et al.
**摘要**: 本研究探讨了WNT10A突变导致外胚层发育不良的机制，通过免疫组织化学和Western blot分析，使用WNT10A抗体证实突变导致蛋白表达减少，影响口腔上皮祖细胞分化和牙釉质形成。
2. **文献名称**: *WNT10A regulates proliferation and differentiation of human epidermal stem cells through β-catenin signaling*
**作者**: Fujimaki, H., et al.
**摘要**: 作者利用WNT10A特异性抗体进行免疫荧光染色，发现WNT10A在表皮干细胞中高表达，并通过β-catenin通路调控细胞增殖和分化，为皮肤再生研究提供依据。
3. **文献名称**: *Development and validation of a polyclonal antibody for specific detection of human WNT10A protein*
**作者**: Böhme, K., et al.
**摘要**: 本研究报道了一种兔源多克隆抗体的制备与验证，通过ELISA、免疫印迹和免疫组化证明其对人WNT10A蛋白的高特异性和敏感性，适用于多种实验模型。
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**注**：以上文献信息为模拟示例，实际引用时请通过PubMed、Google Scholar等平台以关键词“WNT10A antibody”或“WNT10A immunohistochemistry”检索并核实详细信息。
The Wnt family member 10A (WNT10A) antibody is a crucial tool for studying the WNT10A protein, a key component of the evolutionarily conserved Wnt signaling pathway. This pathway regulates embryonic development, cell proliferation, differentiation, and tissue homeostasis. WNT10A specifically binds to Frizzled receptors and co-receptors to activate both canonical (β-catenin-dependent) and non-canonical signaling cascades. It plays vital roles in ectodermal tissue development, including tooth formation, hair follicle morphogenesis, nail growth, and skeletal patterning.
Mutations in the WNT10A gene are linked to human pathologies such as ectodermal dysplasia syndromes (e.g., odonto-onycho-dermal dysplasia), oligodontia (tooth agenesis), and rare skeletal disorders. Dysregulated WNT10A expression has also been implicated in cancers, fibrosis, and metabolic diseases. Researchers utilize WNT10A antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression patterns, subcellular localization, and signaling dynamics in developmental models or disease contexts.
Commercial WNT10A antibodies are typically raised against specific epitopes of human WNT10A, with cross-reactivity varying between species. Validation using knockout controls or recombinant proteins is essential due to potential off-target effects. These antibodies facilitate mechanistic studies of WNT10A's dual role as both a developmental regulator and disease mediator, offering insights for therapeutic targeting. Recent applications include exploring its involvement in stem cell maintenance and tissue regeneration processes.
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