首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠红细胞生成素(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPO含量。EPO（促红细胞生成素）是一种糖蛋白激素，主要在肾脏合成，控制红细胞生成。其研究机制涉及通过JAK2-STAT5通路促进红细胞生成，同时具有神经保护作用，如治疗神经退行性疾病。EPO在类风湿关节炎等疾病的研究中，发现其通过上调神经氨酸酶3（NEU3）表达，降低滑膜成纤维细胞的唾液酸化水平，进而促进疾病进展。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探究贫血发生机制、肾脏功能损伤模型、高原低氧适应性研究以及促红细胞生成类药物开发的科研实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EpoErythropoietin ELISA Kit
缩写：EPO
Uniprot No.：P29676
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of rat EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 95 | 86-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1762.1912.1842.07551.6421.6241.6331.5242.51.2361.2181.2271.1181.250.8420.8590.8510.7420.6250.5370.5220.5300.4210.3120.3730.3810.3770.2680.1560.1540.1620.1580.04900.1080.1100.109 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat EPO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled EPO antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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