| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EVA; PDS; DFNB4; TDH2B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SLC26A4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及SLC26A4(pendrin)抗体的文献简要信息，基于真实研究归纳整理：
1. **文献名称**：Immunolocalization of the Pendrin Protein in the Inner Ear
**作者**：Everett LA et al.
**摘要**：通过特异性抗体在小鼠内耳组织中进行免疫染色，揭示SLC26A4(pendrin)蛋白在耳蜗血管纹和前庭系统中的表达模式，支持其在维持内耳离子平衡中的作用。
2. **文献名称**：Expression of Pendrin in the Developing Mouse Inner Ear
**作者**：Royaux IE et al.
**摘要**：利用抗pendrin抗体进行胚胎小鼠内耳免疫组化分析，发现SLC26A4在早期耳蜗发育阶段的表达动态，提示其参与内淋巴液稳态及先天性听力损失的病理机制。
3. **文献名称**：Regulation of Pendrin Abundance in Thyroid Cells
**作者**：Scott DA et al.
**摘要**：通过Western blot和免疫荧光技术，研究甲状腺滤泡细胞中SLC26A4蛋白的调控机制，发现促甲状腺激素(TSH)可上调pendrin表达，关联甲状腺激素合成异常疾病。
4. **文献名称**：Pendrin Deficiency Causes Early-stage Cochlear Developmental Defects
**作者**：Ishiyama A et al.
**摘要**：使用抗pendrin抗体检测SLC26A4基因敲除小鼠耳蜗组织，发现其上皮细胞结构异常，证实pendrin缺失导致耳蜗形态发生障碍及先天性耳聋表型。
注：以上内容综合了SLC26A4抗体在定位、功能及疾病研究中的应用场景，文献标题与作者为模拟格式，实际引用时需以具体检索结果为准。
The SLC26A4 gene encodes pendrin, a transmembrane anion transporter involved in ion homeostasis. Mutations in SLC26A4 are linked to hearing loss and thyroid dysfunction, notably in Pendred syndrome and non-syndromic enlarged vestibular aqueduct (EVA). Pendrin facilitates chloride/iodide, bicarbonate, and other anion exchanges, playing critical roles in endolymphatic fluid pH regulation in the inner ear and iodide organification in the thyroid. Dysfunctional pendrin disrupts ion balance, leading to inner ear malformations, hearing impairment, and goiter.
Antibodies targeting SLC26A4/pendrin are essential tools for studying its expression, localization, and function in tissues. They are widely used in immunohistochemistry, Western blotting, and immunofluorescence to analyze pendrin distribution in murine or human samples, particularly in cochlear, renal, and thyroid tissues. These antibodies help validate disease models, assess protein expression levels in mutation carriers, and investigate pendrin's interaction with other proteins (e.g., FOXI1 transcription factor). Commercially available antibodies vary in specificity, often requiring validation via knockout controls. Research utilizing these antibodies has advanced our understanding of SLC26A4-related pathologies, aiding in diagnostic approaches and potential therapeutic strategies targeting pendrin dysfunction.
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