首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人A型肝配蛋白受体4(EPHA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EPHA4含量。EPHA4是一个重要靶点。它属于Eph受体家族，在神经系统发育中发挥关键作用，参与轴突导向、神经元迁移等过程。研究表明其异常激活或表达与肿瘤、神经系统疾病等多种病理机制相关，目前正探索通过抑制或调节它来开发针对性治疗策略。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研中探究EPHA4在神经系统疾病模型、肿瘤微环境调控或再生医学领域的表达特征，亦可用于药物干预实验或信号通路研究中靶点蛋白的动态监测，为探索EPHA4在细胞通讯、疾病机制及治疗靶点筛选中的功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cek 8 ELISA Kit; CEK8 ELISA Kit; EK8 ELISA Kit; eph receptor a4 ELISA Kit; EPH-like kinase 8 ELISA Kit; EPHA4 ELISA Kit; EPHA4_HUMAN ELISA Kit; Ephrin type-A receptor 4 ELISA Kit; HEK 8 ELISA Kit; hEK8 ELISA Kit; Receptor protein-tyrosine kinase HEK8 ELISA Kit; Sek 1 ELISA Kit; SEK ELISA Kit; TYRO 1 protein tyrosine kinase ELISA Kit; TYRO1 ELISA Kit; Tyrosine-protein kinase receptor SEK ELISA Kit; Tyrosine-protein kinase TYRO1 ELISA Kit
缩写：EPHA4
Uniprot No.：P54764
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 85-89 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human EPHA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 87 | 85-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.3282.3682.3482.2148001.5511.5981.5751.4414001.0041.0381.0210.8872000.5620.5750.5690.4351000.3970.3810.3890.255500.2650.2780.2720.138250.2010.2090.2050.07100.1350.1320.134 | ||||||
货期：3-5 working days
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