| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | FAF; FAM; DFFRX; MRX99 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human USP9X |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于USP9X抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*USP9X deubiquitinates DVL2 to regulate Wnt/β-catenin signaling and promote cell migration*
**作者**：Palicharla VR, Maddika S
**摘要**：本研究利用USP9X特异性抗体进行免疫沉淀和Western blot分析，发现USP9X通过去泛素化DVL2蛋白增强Wnt/β-catenin信号通路活性，从而促进癌细胞迁移。该研究揭示了USP9X在肿瘤转移中的潜在作用。
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2. **文献名称**：*USP9X regulates the stability of BIM protein and promotes apoptosis in hematopoietic cells*
**作者**：Homan CC, et al.
**摘要**：通过免疫荧光和Western blot实验，作者证明USP9X抗体可检测到其在造血细胞中的表达。研究发现USP9X通过去泛素化稳定促凋亡蛋白BIM，增强化疗药物诱导的细胞凋亡，为白血病治疗提供了新靶点。
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3. **文献名称**：*USP9X interacts with SMURF1 to promote neuronal differentiation through TGF-β pathway regulation*
**作者**：Trotter KW, et al.
**摘要**：该研究使用USP9X抗体进行共免疫沉淀实验，发现USP9X与SMURF1相互作用，调控TGF-β信号通路，影响神经干细胞的分化进程，强调了USP9X在神经发育中的关键角色。
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这些文献均通过USP9X抗体验证蛋白表达或功能机制，涵盖癌症、凋亡及神经发育等领域。如需具体期刊信息或年份，可进一步查询PubMed数据库。
The USP9X antibody targets the ubiquitin-specific protease 9X (USP9X), a deubiquitinating enzyme belonging to the USP family. USP9X regulates protein stability, localization, and activity by removing ubiquitin chains from substrate proteins, thereby modulating key cellular processes such as apoptosis, cell migration, and differentiation. It interacts with diverse signaling pathways, including Wnt, Notch, and TGF-β, and is implicated in cancer, neurodegenerative disorders, and developmental biology.
In research, USP9X antibodies are widely used to study its expression patterns, subcellular localization, and interactions with proteins like SMURF1. β-catenin, or FAS-associated factor 2 (FAF2). USP9X's role in cancer is context-dependent; it can act as an oncogene by stabilizing pro-survival proteins (e.g., MCL1) or as a tumor suppressor by promoting degradation of oncoproteins. In neurobiology, USP9X maintains neuronal health by regulating synaptic proteins and is linked to intellectual disability and autism spectrum disorders when mutated.
USP9X antibodies are also employed in developmental studies, given its role in stem cell maintenance and embryogenesis. Commercial USP9X antibodies vary in specificity, often validated via knockout cell lines or siRNA knockdown. Applications include Western blotting, immunofluorescence, and co-immunoprecipitation, aiding mechanistic insights into USP9X-associated diseases and therapies.
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