| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1CA |
| Uniprot No | P62136 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-330aa |
| 氨基酸序列 | SDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1CA重组蛋白的3篇文献示例(注：以下为模拟内容，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: *"Expression and purification of recombinant human PP1 catalytic subunit alpha (PPP1CA) in Escherichia coli"*
**作者**: Smith J, et al.
**摘要**: 报道了利用大肠杆菌系统高效表达并纯化具有酶活性的重组PPP1CA蛋白，优化了表达条件及纯化步骤，并通过磷酸酶活性实验验证其功能。
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2. **文献名称**: *"Structural insights into the regulatory mechanism of PPP1CA by inhibitor-2"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析了重组PPP1CA蛋白与其调控蛋白Inhibitor-2的复合物结构，揭示了其活性调控的分子机制，为靶向PPP1CA的药物设计提供了结构基础。
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3. **文献名称**: *"Functional characterization of PPP1CA mutations in cancer cell proliferation"*
**作者**: Chen L, et al.
**摘要**: 利用重组PPP1CA蛋白及突变体，研究其在肿瘤细胞周期中的调控作用，发现特定位点突变导致磷酸酶活性改变，进而影响细胞增殖和凋亡通路。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“PPP1CA recombinant protein”获取最新研究。
**Background of PPP1CA Recombinant Protein**
PPP1CA (Protein Phosphatase 1 Catalytic Subunit Alpha) is a key member of the serine/threonine phosphatase family, playing a critical role in regulating diverse cellular processes. This enzyme catalyzes the dephosphorylation of target proteins, counterbalancing kinase activities to maintain cellular homeostasis. The PPP1CA subunit combines with regulatory proteins (e.g., PPP1R) to form holoenzymes, which dictate substrate specificity, localization, and activity. Its involvement spans cell cycle progression, signal transduction, metabolism, and apoptosis, making it essential for normal cellular function.
Recombinant PPP1CA protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and enzymatic activity. This engineered protein retains the native structure and function, enabling researchers to study PP1-mediated mechanisms *in vitro*. Applications include enzyme kinetics, inhibitor screening, and mapping phosphorylation-dependent pathways. Dysregulation of PPP1CA is linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, highlighting its therapeutic relevance.
Studies using recombinant PPP1CA have advanced understanding of PP1's role in DNA repair, synaptic plasticity, and metabolic regulation. Its utility as a tool for probing phosphatase networks underscores its importance in both basic research and drug discovery. By modulating PPP1CA activity, scientists aim to develop targeted therapies for pathologies driven by aberrant phosphorylation signaling.
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