| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPM1F |
| Uniprot No | P49593 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-454aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSSGAPQKSSPMASGAEETPGFLDTLL QDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAA ALAHEAVSQLLQTDLSEFRKLPREEEEEEEDDDEEEKAPVTLLDAQSLAQ SFFNRLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLP SFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQPELPTDP EGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQV ILVQQGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNGTLAVSRA IGDVFQKPYVSGEADAASRALTGSEDYLLLACDGFFDVVPHQEVVGLVQS HLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELLEGGNQGE GDPQAEGRRQDLPSSLPEPETQAPPRS |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPM1F重组蛋白的3篇参考文献及简要摘要：
1. **"PPM1F regulates AMPK signaling through mitochondrial Ca²⁺-dependent dephosphorylation"**
*作者：Li Y. et al. (2018)*
摘要：研究通过表达重组人源PPM1F蛋白，发现其通过调控线粒体钙信号依赖性去磷酸化抑制AMPK活性，揭示其在代谢应激中的调节作用。
2. **"Recombinant PPM1F suppresses inflammatory responses by dephosphorylating CaN/NFAT in macrophages"**
*作者：Wang X. et al. (2020)*
摘要：利用重组PPM1F蛋白验证其对钙调神经磷酸酶(CaN)的去磷酸化作用，证明其通过抑制NFAT通路减轻巨噬细胞炎症反应。
3. **"Structural and functional analysis of PPM1F phosphatase domain using recombinant protein expression"**
*作者：Sato R. et al. (2019)*
摘要：通过大肠杆菌系统表达并纯化PPM1F催化结构域重组蛋白，解析其晶体结构，揭示底物结合特异性及酶活性关键位点。
(注：上述文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
**Background of PPM1F Recombinant Protein**
PPM1F (Protein Phosphatase Mg²⁺/Mn²⁺-Dependent 1F), also known as PP2Cβ or POPX2. belongs to the PPM family of serine/threonine phosphatases. It is characterized by its dependence on divalent cations (Mg²⁺ or Mn²⁺) for catalytic activity. Structurally, PPM1F contains a conserved phosphatase domain responsible for dephosphorylating target substrates, regulating diverse cellular processes such as stress response, apoptosis, and immune signaling. Unlike other phosphatases, PPM1F lacks regulatory subunits, relying instead on post-translational modifications or protein interactions for activity modulation.
PPM1F plays a critical role in modulating key signaling pathways. It negatively regulates the Ca²⁺/calmodulin-dependent protein kinase kinase β (CaMKKβ)-AMP-activated protein kinase (AMPK) axis, impacting cellular energy homeostasis. Additionally, it dephosphorylates and inactivates p38 mitogen-activated protein kinase (MAPK), thereby influencing stress-induced apoptosis and inflammatory responses. Its interaction with proteins like TAB1 (TAK1-binding protein 1) further links it to NF-κB and innate immunity regulation.
Dysregulation of PPM1F has been implicated in diseases. In cancer, PPM1F acts as a tumor suppressor in certain contexts by inhibiting cell migration and invasion, though its role varies across cancer types. In neurodegenerative disorders, it may protect neurons by mitigating mitochondrial dysfunction and oxidative stress. Its expression is notably high in tissues like the heart, skeletal muscle, and brain, underscoring tissue-specific functions.
Recombinant PPM1F protein, produced via bacterial or mammalian expression systems, retains enzymatic activity and is widely used to study phosphatase mechanisms *in vitro*. It enables exploration of substrate specificity, inhibitor screening, and structural analysis (e.g., crystallography). Researchers also employ it to dissect PPM1F's role in disease models, aiding therapeutic development targeting phosphatase-related pathways. Overall, PPM1F recombinant protein serves as a vital tool for unraveling the enzyme's biological significance and translational potential.
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