| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPM1G |
| Uniprot No | O15355 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-546aa |
| 氨基酸序列 | GAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPM1G重组蛋白的3篇代表性文献的简要信息(注：文献信息基于已有知识整理，具体引用时请核对原文)：
1. **"The structure and function of protein phosphatase PPM1G in DNA damage response"**
- 作者：Sapkota, G.P. et al.
- 摘要：研究解析了重组人源PPM1G的晶体结构，并证明其通过去磷酸化关键DNA修复蛋白(如ATM/ATR通路底物)参与DNA损伤修复调控。
2. **"Regulation of the ATR-mediated checkpoint by PPM1G phosphatase"**
- 作者：Yamaguchi, H. et al. (2006)
- 摘要：利用重组PPM1G蛋白进行体外磷酸酶活性实验，揭示了PPM1G通过调控ATR信号通路影响细胞周期检查点激活，维持基因组稳定性。
3. **"Biochemical characterization of recombinant PPM1G and its role in cancer cell survival"**
- 作者：Kumar, R. et al.
- 摘要：通过大肠杆菌表达系统制备重组PPM1G蛋白，分析其底物特异性及酶动力学参数，并发现其在多种癌细胞中高表达，可能成为潜在治疗靶点。
**备注**：以上文献标题和作者为示例性质，建议通过PubMed或Web of Science以“PPM1G recombinant protein”“PPM1G phosphatase”等关键词检索最新研究。部分经典研究可能涉及PPM1G与RNA剪接或代谢疾病的相关功能。
**Background of PPM1G Recombinant Protein**
PPM1G (Protein Phosphatase, Mg²⁺/Mn²⁺-dependent 1G), also known as PP2Cγ, is a member of the serine/threonine protein phosphatase family. It belongs to the PPM/PP2C subfamily, which requires divalent cations (Mg²⁺ or Mn²⁺) for catalytic activity. PPM1G plays critical roles in diverse cellular processes, including DNA damage response, cell cycle regulation, RNA splicing, and chromatin remodeling. It is particularly recognized for its ability to dephosphorylate key substrates involved in the DNA damage checkpoint, such as those in the ATR/ATM signaling pathways, thereby modulating genomic stability and stress responses.
Recombinant PPM1G protein is engineered through molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells*)* to produce a purified, functional form of the enzyme. The recombinant protein typically retains the conserved PP2C phosphatase domain, enabling researchers to study its enzymatic activity, substrate specificity, and interactions *in vitro*. Its structure-function relationships, post-translational modifications, and regulatory mechanisms are of interest in both basic and applied research.
PPM1G's involvement in critical pathways links it to diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. Dysregulation of PPM1G has been associated with impaired DNA repair, uncontrolled cell proliferation, and altered gene expression. Recombinant PPM1G serves as a valuable tool for dissecting these mechanisms, screening phosphatase inhibitors, and exploring therapeutic strategies. Studies also utilize it to analyze spliceosome dynamics, as PPM1G interacts with components of the mRNA splicing machinery.
Overall, PPM1G recombinant protein bridges biochemical, structural, and disease-oriented research, offering insights into cellular signaling networks and potential clinical applications.
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