| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPME1 |
| Uniprot No | Q9Y570 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSALEKSMHLGRLPSRPPLPGSGGSQSGAK MRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLL LLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAET MAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMI DVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVS MVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETK KDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDL TIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQ CVFPGC |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PPME1重组蛋白的示例参考文献(注：部分信息为示例性概括，建议通过PubMed等平台核实)：
1. **"PPME1 regulates tumor cell proliferation through PP2A inhibition in colorectal cancer"**
*Lee, J. et al. (2018)*
摘要：研究通过重组PPME1蛋白实验，揭示其通过甲基化修饰PP2A磷酸酶的催化亚基，抑制PP2A活性，从而促进结直肠癌细胞周期进程的分子机制。
2. **"Structural insights into the catalytic mechanism of human PPME1"**
*Xing, Y. et al. (2015)*
摘要：利用重组人源PPME1蛋白进行X射线晶体学研究，解析其三维结构，发现其活性位点关键残基对底物特异性识别的作用，为开发靶向抑制剂提供结构基础。
3. **"Recombinant PPME1 exacerbates Tau hyperphosphorylation in Alzheimer's disease models"**
*Dominguez, M. et al. (2020)*
摘要：通过体外表达重组PPME1.证明其过表达可增强GSK-3β活性，导致Tau蛋白异常磷酸化，提示PPME1在阿尔茨海默病病理中的潜在作用。
4. **"High-yield purification of active PPME1 using a novel E. coli expression system"**
*Schmidt, C. et al. (2019)*
摘要：开发基于大肠杆菌的重组PPME1高效表达纯化方案，通过离子交换层析和尺寸排阻色谱获得高纯度蛋白，并验证其甲基转移酶活性。
注：以上文献信息为模拟概括，实际研究需以具体数据库检索结果为准。
**Background of PPME1 Recombinant Protein**
PPME1 (Protein Phosphatase Methylesterase 1) is a conserved enzyme that regulates protein phosphatase activity by hydrolyzing methyl ester groups. It specifically demethylates the carboxyl-terminal methylation of protein phosphatase 2A (PP2A), a major serine/threonine phosphatase in eukaryotic cells. PP2A methylation influences its substrate specificity, subcellular localization, and interactions with regulatory subunits, thereby modulating critical signaling pathways involved in cell cycle progression, apoptosis, and stress responses.
Recombinant PPME1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for functional studies. Its recombinant form retains enzymatic activity, making it a valuable tool for investigating PP2A regulation, methyltransferase-demethylase dynamics, and downstream signaling cascades. Studies highlight PPME1's role in diseases, including cancer and neurodegenerative disorders, where PP2A dysregulation is implicated. For example, PPME1 overexpression in certain cancers correlates with enhanced cell proliferation and chemoresistance, suggesting its potential as a therapeutic target.
Structurally, PPME1 contains a conserved esterase domain critical for its catalytic function. Research utilizing recombinant PPME1 has elucidated substrate-binding mechanisms and identified small-molecule inhibitors to modulate its activity. Additionally, it aids in studying cross-talk between post-translational modifications (e.g., phosphorylation and methylation) that fine-tune PP2A function.
Overall, recombinant PPME1 serves as a key reagent for dissecting phosphatase regulation and developing strategies to correct PP2A-associated pathologies. Its applications span *in vitro* enzymatic assays, structural biology, and drug discovery, underscoring its significance in both basic and translational research.
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