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产品描述:人补体因子H相关蛋白2(CFHR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CFHR2含量。CFHR2属于补体因子H相关基因家族,位于染色体1上,参与补体调节。其突变与致密性沉积病和非典型溶血性尿毒症综合征有关。研究显示,CFHR2可能通过调节补体系统活性,影响脂质代谢,在免疫调节中发挥重要作用。试剂盒检测范围为6.25 ng/mL-400 ng/mL,适用于体外细胞实验、疾病机制研究及生物标志物探索等科研场景,可为补体相关疾病的分子机制解析、药物靶点验证及治疗策略开发提供精准的定量分析工具,尤其适合免疫学、病理学及转化医学领域的实验室使用本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CFHL2 ELISA Kit; CFHR2 ELISA Kit; Complement factor H related protein 2 ELISA Kit; Complement factor H-related 2 ELISA Kit; Complement factor H-related protein 2 ELISA Kit; DDESK59 ELISA Kit; Factor H-related gene 2 ELISA Kit; FHR 2 ELISA Kit; FHR-2 ELISA Kit; FHR2 ELISA Kit; FHR2_HUMAN ELISA Kit; H factor (complement)-like 3 ELISA Kit; H factor like 3 ELISA Kit; H factor like protein 2 ELISA Kit; H factor-like 3 ELISA Kit; H factor-like protein 2 ELISA Kit; HFL3 ELISA Kit
缩写:CFHR2
Uniprot No.:P36980
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:6.25 ng/mL-400 ng/mL
灵敏度:1.56 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:100 | Average % | 91 | |||||
Range % | 87-95 | ||||||
1:200 | Average % | 96 | |||||
Range % | 90-102 | ||||||
1:400 | Average % | 104 | |||||
Range % | 98-109 | ||||||
1:800 | Average % | 92 | |||||
Range % | 86-98 |
回收率:
The recovery of human CFHR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 101 | 97-105 | |||||
EDTA plasma (n=4) | 94 | 87-98 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected4002.5202.6372.5792.4852002.1242.2052.1652.0711001.4461.5571.5021.408500.9370.9230.9300.836250.4580.4610.4600.36612.50.2710.2830.2770.1836.250.1560.1630.1600.06600.0930.0950.094 |
货期:3-5 working days
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