首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B因子(BF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CFB含量。人B因子（BF）是补体系统中的关键成分，其研究涉及遗传多态性、连锁不平衡参数等，对B细胞活化有重要调控作用。近年研究发现，BF的异常表达与自身免疫性疾病如系统性红斑狼疮（SLE）密切相关，其研究机制主要聚焦于基因启动子调控和细胞因子影响。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研领域对补体系统功能机制的研究，如免疫相关疾病模型构建、药物干预效果评估或炎症反应通路探索等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AHUS4 ELISA Kit; B factor properdin ELISA Kit; BF ELISA Kit; BFD ELISA Kit; C3 proaccelerator ELISA Kit; C3 proactivator ELISA Kit; C3/C5 convertase ELISA Kit; CFAB ELISA Kit; CFAB_HUMAN ELISA Kit; CFB ELISA Kit; Complement Factor B ELISA Kit; Complement factor B Bb fragment ELISA Kit; FB ELISA Kit; FBI12 ELISA Kit; GBG ELISA Kit; Glycine rich beta glycoprotein ELISA Kit; Glycine-rich beta glycoprotein ELISA Kit; H2 Bf ELISA Kit; H2BF ELISA Kit; PBF2 ELISA Kit; Properdin factor B ELISA Kit
缩写：CFB
Uniprot No.：P00751
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 97 | ||||
| Range % | 89-105 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 85-98 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of human BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-101 | ||||
| EDTA plasma (n=4) | 97 | 91-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.2542.3042.2792.149501.8161.8531.8351.705251.5071.4391.4731.34312.51.0421.0721.0570.9276.250.8130.8020.8080.6783.120.4920.4850.4890.3591.560.3020.3000.3010.17100.1320.1280.130 | ||||||
货期：3-5 working days
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