首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板糖蛋白4(CD36)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD36含量。CD36，又称血小板糖蛋白4，是一种清道夫受体，主要存在于血小板、红细胞、单核细胞等细胞中。它能够结合多种配体，如长链脂肪酸、氧化低密度脂蛋白等，参与脂质摄取、免疫识别、炎症反应等过程。研究机制方面，CD36的棕榈酰化修饰对其定位和脂肪酸吸收活性至关重要。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域对CD36蛋白水平的精确分析。支持科研人员探究CD36在肥胖、动脉粥样硬化等疾病模型中的表达变化，或用于药物干预实验的疗效评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd36 ELISA Kit; FatPlatelet glycoprotein 4 ELISA Kit; Adipocyte membrane protein ELISA Kit; Fatty acid translocase ELISA Kit; Fatty acid transport protein ELISA Kit; Glycoprotein IIIb ELISA Kit; GPIIIB ELISA Kit; PAS IV ELISA Kit; PAS-4 ELISA Kit; Platelet glycoprotein IV ELISA Kit; GPIV ELISA Kit; CD antigen CD36 ELISA Kit
缩写：CD36
Uniprot No.：Q07969
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CD36 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 84-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of rat CD36 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 95-110 | ||||
| EDTA plasma (n=4) | 99 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5042.4532.4792.305 102.0401.9221.9811.807 51.2851.3131.2991.125 2.50.7330.7450.7390.565 1.250.4300.4560.4430.269 0.6250.3260.3420.3340.160 0.3120.2450.2510.2480.074 00.1720.1760.174 | ||||||
货期：3-5 working days
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