首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD2相关蛋白(CD2AP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD2AP含量。CD2AP是一种接头蛋白，与阿尔茨海默病（AD）的发病机制密切相关。研究发现，CD2AP在淀粉样前体蛋白（APP）的转运和降解中起调节作用，影响β-淀粉样蛋白（Aβ）的生成和沉积，同时参与tau蛋白的神经毒性。CD2AP的缺失可能通过调节小胶质细胞的吞噬功能和突触完整性来保护大脑，成为AD治疗的新靶点。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于体外研究CD2AP在疾病模型中的表达水平变化，例如肾组织损伤机制研究、神经退行性疾病相关蛋白互作分析，或通过细胞实验探索CD2AP在受体酪氨酸激酶信号通路中的作用，为靶向药物开发及分子机制研究提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adapter protein CMS ELISA Kit; AL024079 ELISA Kit; C78928 ELISA Kit; Cas ligand with multiple SH3 domains ELISA Kit; CD2 associated protein ELISA Kit; CD2-associated protein ELISA Kit; CD2AP ELISA Kit; CD2AP_HUMAN ELISA Kit; CMS ELISA Kit; Mesenchyme to epithelium transition protein with SH3 domains 1 ELISA Kit; METS 1 ELISA Kit; Mets1 ELISA Kit
缩写：CD2AP
Uniprot No.：Q9Y5K6
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD2AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 82-93 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of human CD2AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 82-94 | |||||
| EDTA plasma (n=4) | 93 | 86-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.8642.7552.8102.6946002.1372.2122.1752.0593001.4561.5371.4971.3811500.9560.9460.9510.835750.4970.4890.4930.37737.50.2740.2890.2820.16618.750.2180.2100.2140.09800.1160.1150.116 | |||||||
货期：3-5 working days
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