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产品描述：人可溶性CD28(sCD28)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD28含量。CD28是一种重要的共刺激分子，位于T细胞表面，对T细胞的激活、增殖、分化和存活发挥关键作用。其研究机制涉及与B7-1/B7-2结合，作为“第二信号”降低T细胞活化阈值，强化“第一信号”激活，并参与免疫调节。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本试剂盒适用于科研领域研究sCD28在感染性疾病模型、免疫调控机制或组织微环境中的表达特征。在自身免疫性疾病、慢性炎症及肿瘤微环境研究中具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28
缩写：CD28
Uniprot No.：P10747
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of human sCD28 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.2602.3022.2812.158201.7431.7021.7231.600101.2271.3041.2661.14350.8510.8320.8420.7192.50.5040.4980.5010.3781.250.3110.3010.3060.1830.6250.2060.2000.2030.08000.1210.1240.123 | |||||||
货期：3-5 working days
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