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产品描述：人可溶性CD276分子(sCD276/sB7-H3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的CD276含量。CD276（B7-H3）是一种免疫检查点蛋白，在肿瘤细胞和肿瘤微环境中的免疫细胞中选择性表达。其研究机制表明，CD276与肿瘤细胞增殖、转移和治疗耐药性有关，并可能通过调节T细胞活性促进肿瘤免疫逃逸。CD276作为潜在的治疗靶点，在癌症免疫治疗中具有重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研中定量分析sCD276的表达水平，例如探究肿瘤微环境与免疫细胞的相互作用、炎症性疾病机制研究、药物干预效果评估或生物标志物筛选等场景，为免疫相关疾病机制和转化医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4Ig B7 H3 ELISA Kit; 4Ig-B7-H3 ELISA Kit; AU016588 ELISA Kit; B7 H3 ELISA Kit; B7 homolog 3 ELISA Kit; B7-H3 ELISA Kit; B7H3 ELISA Kit; B7RP-2 ELISA Kit; CD_antigen=CD276 ELISA Kit; CD276 ELISA Kit; CD276 antigen ELISA Kit; CD276 molecule ELISA Kit; CD276_HUMAN ELISA Kit; Costimulatory molecule ELISA Kit; Flags: Precursor ELISA Kit; PSEC0249 ELISA Kit; UNQ309/PRO352 ELISA Kit
缩写：CD276
Uniprot No.：Q5ZPR3
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD276/sB7-H3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human sCD276/sB7-H3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.3982.3872.3932.2401001.9741.9991.9871.834501.3791.3981.3891.236250.8540.8010.8280.67512.50.4860.4720.4790.3266.250.3350.3250.3300.1773.120.2620.2790.2710.11800.1520.1540.153 | ||||||
货期：3-5 working days
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