首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人程序性死亡因子配体1(PD-L1/CD274)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD274含量。CD274即PD-L1，是一种重要的免疫检查点分子。其背景在于机体免疫系统攻击肿瘤时，它可与T细胞表面PD-1结合，抑制T细胞活性，使肿瘤逃逸免疫攻击。研究机制主要围绕阻断二者结合，恢复T细胞功能，以增强机体抗肿瘤免疫反应。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于科研领域中对PD-L1蛋白表达的定量分析，例如评估癌症模型或临床前研究中PD-L1的动态变化、探究免疫治疗药物的作用机制，或分析不同样本类型（如细胞培养上清、组织提取物）中PD-L1的分泌水平。为肿瘤免疫学、药物开发及生物标志物研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B7 H ELISA Kit; B7 H1 ELISA Kit; B7 homolog 1 ELISA Kit; B7-H1 ELISA Kit; B7H ELISA Kit; B7H1 ELISA Kit; CD 274 ELISA Kit; CD274 ELISA Kit; CD274 antigen ELISA Kit; CD274 molecule ELISA Kit; MGC142294 ELISA Kit; MGC142296 ELISA Kit; OTTHUMP00000021029 ELISA Kit; PD L1 ELISA Kit; PD-L1 ELISA Kit; PD1L1_HUMAN ELISA Kit; PDCD1 ligand 1 ELISA Kit; PDCD1L1 ELISA Kit; PDCD1LG1 ELISA Kit; PDL 1 ELISA Kit; PDL1 ELISA Kit; Programmed cell death 1 ligand 1 ELISA Kit; Programmed death ligand 1 ELISA Kit; RGD1566211 ELISA Kit
缩写：CD274
Uniprot No.：Q9NZQ7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PD-L1/CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-107 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human PD-L1/CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-104 | ||||
| EDTA plasma (n=4) | 95 | 90-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2742.1742.2241.9655001.9401.8541.8971.6382501.4501.3551.4031.1441250.9270.9550.9410.68262.50.5700.5800.5750.31631.20.4220.4170.4200.16115.60.3520.3410.3470.08800.2530.2650.259 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD274 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CD274 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
