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产品描述：人二级淋巴组织趋化因子(SLC/CCL21)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CCL21含量。CCL21 作为一种靶点，在生物医学领域备受关注。它属于趋化因子，在淋巴细胞迁移及归巢等过程发挥重要作用。研究机制上，主要围绕其与受体结合，介导免疫细胞的定向移动展开，这对免疫调节、炎症反应等方面研究意义重大。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品为免疫学、肿瘤生物学等领域的基础研究提供可靠工具，可用于探索疾病模型中的免疫细胞迁移机制、评估炎症相关生物标志物动态变化或药物干预对趋化因子网络的影响。适用于实验室常规蛋白定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：6Ckine ELISA Kit; Beta chemokine exodus 2 ELISA Kit; Beta-chemokine exodus-2 ELISA Kit; C C motif chemokine ligand 21 ELISA Kit; C-C motif chemokine 21 ELISA Kit; CCL21 ELISA Kit; CCL21_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 21 ELISA Kit; Chemokine CC motif ligand 21 ELISA Kit; CKb9 ELISA Kit; ECL ELISA Kit; Efficient Chemoattractant for Lymphocytes ELISA Kit; SCYA21 ELISA Kit; Secondary lymphoid tissue chemokine ELISA Kit; Secondary lymphoid-tissue chemokine ELISA Kit; SLC ELISA Kit; Small inducible cytokine A21 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys); member 21 ELISA Kit; Small-inducible cytokine A21 ELISA Kit; TCA4 ELISA Kit; UNQ784/PRO1600 ELISA Kit
缩写：CCL21
Uniprot No.：O00585
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SLC/CCL21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 92 | ||
| Range % | 89-98 | |||
| 1:2 | Average % | 103 | ||
| Range % | 95-107 | |||
| 1:4 | Average % | 91 | ||
| Range % | 86-98 | |||
| 1:8 | Average % | 93 | ||
| Range % | 87-98 |
回收率：
| The recovery of human SLC/CCL21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 91-102 | |||||
| EDTA plasma (n=4) | 86 | 82-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.9832.9912.9872.844 25002.2172.1832.2002.057 12501.4371.4891.4631.320 6250.8900.8830.8870.744 312.50.5580.5760.5670.424 1560.3990.3820.3910.248 780.2570.2520.2550.112 00.1420.1430.143 | |||||||
货期：3-5 working days
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