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产品描述：人钙蛋白酶2（M / II）大亚基（CAPN2）(CAPN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的CAPN2含量。CAPN2是一种钙激活中性半胱氨酸蛋白酶，在多种细胞活动中发挥作用。其背景在于与细胞凋亡、信号传导等密切相关。研究机制主要围绕其在疾病发生发展进程中的作用，如在肿瘤转移、神经退行性疾病等方面的潜在影响备受关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于细胞生物学、分子病理学等领域的基础研究，例如探究CAPN2在疾病模型中的表达动态、药物干预对蛋白酶活性影响等实验场景，为揭示其生理功能及调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Calcium activated neutral proteinase 2 ELISA Kit; Calcium activated neutral proteinase ELISA Kit; Calcium-activated neutral protease 2; catalytic subunit ELISA Kit; Calcium-activated neutral proteinase 2 ELISA Kit; CALP80 ELISA Kit; Calpain 2 (m/II) large subunit ELISA Kit; Calpain 2 catalytic subunit ELISA Kit; Calpain 2 large catalytic subunit ELISA Kit; Calpain 2 large subunit ELISA Kit; Calpain 2; large [catalytic] subunit ELISA Kit; Calpain large polypeptide L2 ELISA Kit; Calpain M type ELISA Kit; Calpain M-type ELISA Kit; Calpain-2 catalytic subunit ELISA Kit; Calpain-2 large subunit ELISA Kit; Calpain2 ELISA Kit; CAN2_HUMAN ELISA Kit; CANP 2 ELISA Kit; CANP L2 ELISA Kit; CANP2 ELISA Kit; CANPL 2 ELISA Kit; CANPL2 ELISA Kit; CANPml ELISA Kit; Capa2 ELISA Kit; CAPN 2 ELISA Kit; CAPN2 ELISA Kit; FLJ39928 ELISA Kit; M calpain ELISA Kit; M calpin ELISA Kit; M type ELISA Kit; M-calpain ELISA Kit; mCANP ELISA Kit; Millimolar calpain ELISA Kit; Millimolar-calpain ELISA Kit
缩写：CAPN2
Uniprot No.：P17655
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 84-90 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 98-104 | ||||||
回收率：
| The recovery of human CAPN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.4322.5022.4672.327 51.6881.6981.6931.553 2.50.9230.9340.9290.789 1.250.4890.4760.4830.343 0.6250.3290.3190.3240.184 0.3120.2560.2620.2590.119 0.1560.2010.2100.2060.066 00.1390.1410.140 | |||||||
货期：3-5 working days
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