| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DKCB2; NHP2P; NOLA2 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NHP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NHP2抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用需根据真实文献调整)：
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1. **文献名称**: *"NHP2 Antibody Characterization in Telomerase Complex Assembly"*
**作者**: Smith A, et al.
**摘要**: 本研究利用兔源多克隆NHP2抗体，通过免疫共沉淀和Western blot技术，揭示了NHP2在人类端粒酶复合体组装中的关键作用，证实其与dyskerin蛋白的功能性互作。
2. **文献名称**: *"Monoclonal Anti-NHP2 Antibody as a Biomarker in Colorectal Cancer"*
**作者**: Zhang Y, et al.
**摘要**: 开发了一种高特异性小鼠单克隆NHP2抗体，用于免疫组化分析。结果显示，结直肠癌组织中NHP2蛋白显著高表达，提示其可能作为新型肿瘤诊断标志物。
3. **文献名称**: *"Autoantibodies Against NHP2 in Systemic Lupus Erythematosus"*
**作者**: Lee S, et al.
**摘要**: 通过ELISA检测系统性红斑狼疮(SLE)患者血清，发现抗NHP2自身抗体的存在与疾病活动度相关，为自身免疫病的分子机制研究提供了新方向。
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**备注**：以上文献为示例性质，实际研究中请通过PubMed或Google Scholar检索真实发表的论文，关键词建议为“NHP2 antibody”、“anti-NHP2”或“NHP2 autoantibody”。
The NHP2 antibody is a crucial tool in studying the NHP2 protein, a component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex. NHP2. along with proteins like dyskerin, GAR1. and NOP10. plays a vital role in ribosomal RNA (rRNA) processing, telomerase RNA stabilization, and post-transcriptional modifications such as pseudouridylation. This complex is essential for ribosome biogenesis and telomere maintenance, linking NHP2 dysfunction to genetic disorders like dyskeratosis congenita (DKC), a condition characterized by bone marrow failure, skin abnormalities, and increased cancer risk.
Antibodies targeting NHP2 are widely used in molecular and cellular biology to investigate its expression, localization, and interactions within the H/ACA complex. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence, helping researchers dissect NHP2's role in ribosome assembly, telomere biology, and disease mechanisms. Studies using these antibodies have revealed how mutations in NHP2 disrupt rRNA processing and telomerase activity, contributing to DKC pathogenesis and cancer progression. Additionally, NHP2 antibodies are valuable in exploring connections between ribosomopathies, aging-related disorders, and telomere-associated diseases. Their application extends to diagnostic research, aiding in the identification of molecular biomarkers for early disease detection or therapeutic targeting.
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