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产品描述：人腺苷酸环化酶关联蛋白1(CAP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CAP1含量。CAP1是一种与腺苷酸环化酶相关的蛋白，参与细胞骨架的调节和细胞迁移。其在mRNA稳定性、翻译和免疫识别中起关键作用，与动脉粥样硬化、内皮细胞衰老等疾病相关。研究CAP1有助于揭示相关疾病的发病机制，为疾病治疗提供新靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品可广泛应用于基础医学研究领域，例如探究CAP1在代谢紊乱模型中的动态变化、评估炎症相关信号通路的调控机制，或作为肿瘤微环境研究的辅助工具。实验流程兼容常规酶标仪分析，为研究者提供稳定可靠的定量数据支持，满足细胞生物学、病理机制探索等科研场景的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adenylyl cyclase associated protein 1 ELISA Kit; Adenylyl cyclase-associated protein 1 ELISA Kit; CAP 1 ELISA Kit; CAP ELISA Kit; CAP; adenylate cyclase associated protein 1 (yeast) ELISA Kit; CAP1 ELISA Kit; CAP1 PEN ELISA Kit; CAP1-PEN ELISA Kit; CAP1_HUMAN ELISA Kit
缩写：CAP1
Uniprot No.：Q01518
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-99 | |||||
回收率：
| The recovery of human CAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-93 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.1042.2682.1862.0838001.5781.5901.5841.4814001.1631.1521.1581.0552000.7070.7270.7170.6141000.4950.4740.4850.382500.2920.2780.2850.182250.1960.1890.1930.09000.1020.1040.103 | ||||||
货期：3-5 working days
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