首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人抗菌肽(CAMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CAMP含量。CAMP（环腺苷酸）是细胞内重要的第二信使，参与多种生理过程。研究显示，失控的cAMP可能导致肝癌，其机制涉及cAMP在无膜细胞器中的动态隔离，当这些结构被破坏时，cAMP会淹没细胞，导致失控生长。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于体外科研实验中探索CAMP在感染性病原体应答、慢性炎症性疾病（如银屑病、炎症性肠病）或皮肤创伤愈合等生理病理过程中的表达变化，为免疫机制研究及潜在生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
缩写：CAMP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 88 | |
| Range % | 80-92 | ||
| 1:200 | Average % | 96 | |
| Range % | 91-105 | ||
| 1:400 | Average % | 99 | |
| Range % | 92-110 | ||
| 1:800 | Average % | 95 | |
| Range % | 86-98 |
回收率：
| The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 302.7132.6642.6892.612 151.6871.5671.6271.550 7.51.0470.9891.0180.941 3.750.5630.5430.5530.476 1.80.3390.3240.3320.255 0.90.2060.1960.2010.124 0.450.1450.1390.1420.065 00.0800.0740.077 | ||||||
货期：3-5 working days
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