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产品描述：人不典型蛋白C9orf72(C9orf72)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的C9orf72含量。C9orf72是一个重要的研究靶点。它与肌萎缩侧索硬化症和额颞叶痴呆等神经退行性疾病相关。研究机制围绕其六核苷酸重复扩增，这会引发RNA毒性、二肽重复蛋白聚集等病理过程，从而影响神经细胞功能和生存。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒为研究C9orf72蛋白在疾病模型中的表达变化、探索神经退行性病变分子机制、评估实验性治疗干预效果等基础研究提供可靠工具。适用于细胞生物学、神经科学等领域的蛋白定量分析需求，仅限科研使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALSFTD ELISA Kit; C9orf72 ELISA Kit; chromosome 9 open reading frame 72 ELISA Kit; CI072_HUMAN ELISA Kit; FTDALS ELISA Kit; MGC23980 ELISA Kit; Protein C9orf72 ELISA Kit; RP11-27J8.2 ELISA Kit; Uncharacterized protein C9orf72 ELISA Kit
缩写：C9orf72
Uniprot No.：Q96LT7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C9orf72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of human C9orf72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-89 | ||||
| EDTA plasma (n=4) | 91 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2772.3982.3382.23110001.9471.9301.9391.8325001.3931.3751.3841.2772500.9110.9350.9230.8161250.6050.6410.6230.51662.50.4010.4320.4170.31031.250.2880.2980.2930.18600.1060.1080.107 | ||||||
货期：3-5 working days
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