首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体成分C8β链(C8B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C8B含量。人补体成分C8β链（C8B）是补体系统的一部分，参与免疫反应和炎症过程。其研究机制涉及C8β链与C5b67结合，激活C8αγ，进而参与膜攻击复合物的形成，对病原体进行杀伤。试剂盒检测范围为2.74 pg/mL-2000 pg/mL，其标准化操作流程支持科研人员探究补体系统在感染、炎症或自身免疫性疾病中的作用机制，例如通过分析不同病理条件下血清或组织中C8β链的表达差异，揭示补体激活途径的调控规律。此外，还可用于评估药物干预或基因编辑模型对补体相关通路的影响，为免疫学基础研究及转化医学提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C8b ELISA Kit; CO8B_HUMAN ELISA Kit; Complement component 8 beta polypeptide ELISA Kit; Complement component 8 subunit beta ELISA Kit; Complement component C8 beta chain ELISA Kit
缩写：C8B
Uniprot No.：P07358
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：2.74 pg/mL-2000 pg/mL
灵敏度：1.37 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:10000 | Average % | 92 | |||||
| Range % | 89-98 | ||||||
| 1:20000 | Average % | 90 | |||||
| Range % | 84-100 | ||||||
| 1:40000 | Average % | 95 | |||||
| Range % | 92-101 | ||||||
| 1:80000 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human C8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 85-104 | |||||
| EDTA plasma (n=4) | 91 | 88-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.5562.5102.5332.442 6671.8811.8161.8491.758 2221.2531.3011.2771.186 740.9080.8960.9020.811 24.70.3740.3660.3700.279 8.20.2050.1950.2000.109 2.740.1410.1340.1380.047 00.0890.0930.091 | |||||||
货期：3-5 working days
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